Unit 2- Practical Scientific Procedures and Techniques
Exam (elaborations)
BTEC Applied Science: Unit 2 Calorimetry
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Unit 2- Practical Scientific Procedures and Techniques
Institution
PEARSON (PEARSON)
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Unit 2- Practical Scientific Procedures and Techniques
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Colorimetry
Calibration of a colorimeter
Abstract
It is critical to calibrate the colorimeter before beginning the colorimetry experiment. This is
done to ensure that we will have accurate and exact data at the conclusion of the
experiment. Calibration is an excellent method of ensuring the most accurate and exact
results.
Materials
Cuvette
Distilled water
Colorimeter
Method
1. Fill a cuvette with distilled water and cap it, make sure to only touch it by the top of
the cuvette.
2. Holding the cuvette by the lid, wipe the outside of the cuvette with a non-abrasive
cleaning tissue.
3. Place cuvette into the colorimeter and close the lid.
4. Push calibrate button on the colorimeter.
5. The colorimeter is now calibrated.
Using colorimetry to find the absorbance of copper sulphate
Abstract
The Beer-Lambert law, which states that the concentration of a solute is proportional to its
absorbance, was used in this experiment to measure the concentration of coloured
compounds in solutions. During this experiment, we will be utilising a colorimeter, which is
used to measure the absorption of light waves. We will begin by calibrating the colorimeter,
which will allow us to obtain correct findings from the experiment. Next, we will be finding
the concentration of the different coloured compounds in solutions by the application of
Beer-Lamberts Law. The data we received was then put on a graph and shown to be correct.
The colorimetry approach was demonstrated to be particularly successful in determining the
concentration of coloured substances.
Introduction
We had employed the use of colorimetry to determine the concentration of an unknown
copper sulphate solution. Light from an LED light source will flow through the solution and
strike a photocell in this device. This experiment's CuSO4 solution is a rich blue tint. A
colourful solution with a higher concentration absorbs lighter and transmits less light than a
solution with a lower concentration. The light received by the photocell will be interpreted
by the colorimeter and expressed as an absorbance and a percent transmittance value. We
shall make five known concentrations of copper sulphate solutions (standard solutions).
Each solution will be transferred to a tiny, rectangular cuvette, which will then be put in the
colorimeter. Each solution's absorbance will be measured and documented. A graph of
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