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Samenvatting Natuurkunde Course 3
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Dit is de samenvatting voor natuurkunde course 3, leerjaar 1.
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Natuurkunde Course 3Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of
-macromolecules (NDA, RNA and proteins) and their fragments
-based on their size and charge under influence of an electric field
Zone electrophoresis
-paper electrophoresis
serum proteins
-gel electrophoresis
protein, DNA, RNA
-thin layer electrophoresis
amino acids
-cellulose acetate electrophoresis
clinical diagnostics: haemoglobin, serum proteins for monoclonal gammapathies, urine
proteins, isoenzymes, lipo and glycoproteins.
Moving boundary electrophoresis
-capillary electrophoresis
forensic sciences: DNA, ink
-isotachophoresis
analytical chemistry: separation/ characterisation ionic compounds
-isoelectric focussing
protein separation on isoelectic point (pl)
-immuno electrophoresis
protein separation/ characterisation using antibodies
,Electrophoresis
Necessary for electrophoresis
Electric field, electrodes
Matrix, polyacrylamide or agarose
Buffer, electrolyt
Charged bio-molecules
Charged biomolecules, this depends on:
-pH value
-effective charge
Others ions which are bound can shield the charge of the biomolecule
Size
Depends on hydrated radius bounded water-molecules dipole-ion bond
, Motion and velocity
Motion can be uniformly
-velocity v is constant there will be no motion acceleration
Uniformly accelerated
-motion velocity v is not constant there will be a motion acceleration
*highly charged molecules move faster towards the electrode than molecules with a lesser
charge!
*smaller molecules migrate faster than large molecules!
Force
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