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Observing stages of mitosis
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Full CPAC 5 write up which achieved passing grade and fulfilled all necessary criteria for Biology Edexcel
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(Core Practical ____5____ )Title _________Observing mitosis in root tip squash____________
METHOD
1. Carry out instructions given (or those you have produced and researched) to complete the
practical (CPAC1)
2. List apparatus below and describe techniques used when measuring quantities with a
particular piece of apparatus:
Piece of apparatus Purpose of piece of Comment on how piece of equipment Appendix
apparatus was used 5c
accurately/appropriately/safely technique
Microscopes The microscope was used It was used to observe the root tip
to observe and study cells of the garlic. To make sure it was
small objects (the cells used safely, the microscope was kept
and their organelles) that away from the edge of the table. It
cannot be seen to the was also kept away from any fluids
naked eye. (water) as it is electrical. For accuracy,
it was put to the correct resolution
and magnification.
forceps It was used to pick up the It was used gently to ensure it doesn’t
root tips to place them break up the fragile root tips
elsewhere
pipettes I used them to measure Hydrochloric acid 2cm3 was required
and transfer water and for the experiment and the pipette
liquids, used to get 2cm3 was required to gain this correct
acid and for dye amount
Toluidine blue dye The dye was used to stain I ensured the dye did not stay for too
the cells so that the long or too short or else observing the
features can be seen cells would be difficult
more clearly 2-3 drops for 2 minutes
Microscopes slides They were used to place Coverslip was to be dealt with
and coverslip the root tip on to observe carefully and we used a pencil and
under the microscope tissue to press down to ensure it didn’t
break
Mounted need It was used during the It was done slowly to ensure the root
maceration of root tip tip did not break apart
(CPAC a2a, 2b, 2d, 3a, 3b)
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