Concepts Of Protein Technology And Applications (2050FBDBMW)
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Summary and notes on the chapter “Amino acid analysis and sequencing” of (Concepts of Protein technology and applications: Partim Boonen)
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Course
Concepts Of Protein Technology And Applications (2050FBDBMW)
Institution
Universiteit Antwerpen (UA)
This summary includes the summary and notes on the chapter “Amino acid analysis and sequencing” from the part of Concepts of Protein technology and applications taught by professor Boonen. A separate document is uploaded for the part taught by professor Van Ostade. Both parts need to be known f...
Aa_sequencing (part kurt boonen, amino acid analysis and sequencing, concepts of protein technology and applications)
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applications
technology
concepts of protein
protein
concepts
boonen
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kurt boonen
msms
proteomics
concepts
concepts of protein technology and applications
ms
concepts of protein technology and appl
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Concepts Of Protein Technology And Applications (2050FBDBMW)
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Amino acid analysis
Receptor Biology Lab
University of Antwerp
Prof. Stuart Maudsley
, AA analyses and AA sequencing
are two different techniques
AA analysis – methodology used to determine the
AA composition or content of proteins,
peptides and pharmaceutical preparations
AA sequencing – identifies the exact primary
AA sequence of a protein/peptide
,What can Amino Acid Analysis (AAA) do for you ?
Can give you an exact knowledge of protein AA quantities =
required for further protein chemistry applications.
Allows measurement of relative amino acid composition of proteins and
complex protein mixtures
Facilitates measurement of free amino acids in media/fluids
Estimate amounts of contaminating protein in DNA and other non-protein
samples
Very accurate quantification: E.g. isotopically labeled internal standards
used for absolute quantification in LC-MS are calibrated by AAA
, What can Amino Acid Analysis (AAA) do for you ?
Facilitates measurement of free amino acids in media/fluids, e.g. in PKU
PKU = phenylketonuria, a genetic metabolic disease
During development PKU children cannot ingest Phe
(potentially life-threatening). Thus detection of Phe in
food/drugs is vital
normal
PKU
Phenylalanine
hydroxylase
deficiency
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