Other
ILT 3 Report
- Course
- Institution
ILT3 report for BBS2001
[Show more]
Seller
Follow
Maastrichtstudent23
Reviews received
Content preview
ILT 3 Report GMO plasmid DNA isolation 2022/2023Goal:
The purpose of this laboratory practice was to try and isolate the plasmid which was given to us, these
GMOs were E. Coli or more precisely, DH5 with pcDNA 3.1(+) empty vector.
Our goal was to try and isolate this pcDNA 3.1(+) and by using restriction enzymes also trying to cut this
DNA and hoping that this was visible in the gel electrophoresis.
Results:
In the 3rd lane you can see the isolated pcDNA3.1 (+) ,which has a lot of large DNA bands and maybe a
few or a very low amount of small DNA bands. (The glass was not very clear).
In the control digest pcDNA 3.1 (+) (Lane 4) you can see very nice that is has been cut as there are more
smaller DNA bands and no large DNA bands.
In the isolated and digested pcDNA 3.1 (+) (Lane 5) you can clearly see both details of the control digest
and the isolated pcDNA 3.1 (+). So, this was done successfully.
i. Lane 1: LHE ladder
ii. Lane 2: Control pcDNA 3.1 (+)
iii. Lane 3: Isolated pcDNA 3.1 (+)
iv. Lane 4: Control pcDNA 3.1 (+) digest Dral
v. Lane 5: Isolated pcDNA 3.1 (+) digest Dral
Image 1 Image 2 Image 3
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Maastrichtstudent23. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $6.51. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75057 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now