ELISA Worksheet -The Enzyme Linked Immunosorbent Assay
ELISA Worksheet – Please go to Assignments to Download and submit this worksheet
Enzyme-Linked Immunosorbent Assay (ELISA) uses an enzyme-linked indicator (color change) antibody to visualize
antigen-antibody reactions. It is done in a mic...
The Enzyme Linked Immunosorbent Assay (ELISA)
ELISA Worksheet – Please go to Assignments to Download and submit this worksheet
Enzyme-Linked Immunosorbent Assay (ELISA) uses an enzyme-linked indicator (color change) antibody to visualize
antigen-antibody reactions. It is done in a microtiter plate with wells that can adsorb the reactions. It is a technique that
can be used to detect antigens or antibodies in a patient’s serum, thus identifying the presence or history of infection
with a microorganism.
Watch the recorded lecture on the ELISA under Course Content and watch the video demonstrating the direct and
indirect ELISA at https://www.youtube.com/watch?v=lUWpWKVcmc4&t=17s . Use the information in these resources
to answer the following questions
1. Describe the following methods of identifying microbes or for diagnosing infections:
a. Phenotypic method:
Relies on the physical appearance or the microbe
b. Genotypic method:
It relies on information on genetic makeup of the organism
c. Immunologic method: it relies on the chemical make of the microbe.
2. Describe the two sides to the immune system:
The body's initial line of defense against infections is innate immunity. It is broad and non-specific, meaning it does not
distinguish across pathogen kinds. Adaptive immunity is a form of immunity which develops as a result of disease
exposure or vaccination.
3. Antibodies are _ protective proteins_______ that function to _kill_______________ or
_inactivate_________________ antigens.
4. Antibodies are found in serum. Serum is: _ describe the watery component of blood that's left behind when
blood clots or coagulates__.
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