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Summary All Criteria BTEC Level 3 Unit 2 Assignment 3
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Practical Scientific Procedures and Techniques Assignment 3 Chromatography all criteria and distinction received.
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Unit 2 Assignment 3 chromatographyMoreen mero
How the separation of mixtures using paper chromatography works:
the paper chromatography is used to separate mixtures of resolvable substances, such as
colouring agents in food, and will show the mixture as coloured compounds, where each
component of the mixture, will move at different rates on the paper when the solvent it placed
in soaks up the paper. This will cause the mixture to separate out into coloured different
components, and each has different rate. Whereas chromatography has two different phases:
the stationary phase, that does not move, which is the paper. And the mobile phase, that does
move, which is the solvent. The mobile phase will move and pass up through the stationary
phase taking and carrying different substances with it. Moreover, these dissolved and
different substances in the mixture, are attracted to these two phases, in different properties,
and this will cause the substances to more each at different rate.
Separation of plant pigments in spinach:
In this experiment, paper chromatography was used to separate the plant pigments in spinach.
The Rf values
Rf value is the distance that the spot of the colour has
travelled from the line at the start, and it is divided by
the distance that the solvent has travelled.
Rf = distance travelled by substance / distance
travelled by solvent
Rf values calculated for spots on the chromatogram:
Yellow: 7..5 = 0.7
Light green: .5 = 0.8
Green: 10..5 = 0.9
Most of the Rf values are quite high, which indicates
that the substance are non-polar. Meaning that the
substances moved mostly the entire distance the solvent
has travelled to. The higher the Rf values, means the
substance is non-polar, the lower the Rf value, means
the substance is polar.
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