Summary
Summary Unit 6 Investigative project Assignment 3-Chromatography results
- Course
- Institution
Unit 6 Investigative project Assignment 3-Chromatography results , distinction received.
[Show more]
2 reviews
By: delviadominic2004 • 1 year ago
By: mailmaryam04 • 1 year ago
Seller
Follow
missmsnotes
Reviews received
Content preview
/ U6A3 / Chromatography experiment results with expensive and cheap felt-tip pensA study on investigating the difference between expensive and inexpensive felt-tip pens using
chromatography
The lab report:
Introduction:
The production of these pens’ inks is a significantly complicated process: which includes fillers, light stabilizers,
solvents, dyes, and so. Moreover, these felt-tip pens are quite different from ordinary ink pens: this is because
they are highly sensitive to light exposure, and this would cause chromatic or colour fading. However, do all the
felt-tip pen brands use the same components and pigments to create their felt-tip pens? In this project, we are
going to investigate the characterization of the chemicals in types of different felt tip pens brands, using the
chromatography technique. This would allow us to observe the building complex mixtures of the artificial dyes.
Chromatography is a very useful method, it comes in different types; there is thin-layer chromatography, gas
chromatography, paper chromatography, and so. And Each will be carried out through a different system or
material: a sheet, a column, or a plate. And it is called the stationary phase. And fluid is used to separate a mixture
into different substances, this liquid of solvent is called the mobile phase. Each substance of a mixture has different
properties, and this would show on the stationary phase. As each substance, or element would travel at a certain
distance depending on the connections it has with the surface of the stationary phase. For this investigation, paper
chromatography will be used, when the paper would absorb the substances of the felt-tip pen’s ink, and the
separation would be shown as different colours on the paper at different spots.
The procedure:
The equipment :
- 2 beakers ( 400 ml3 )
- Chromatography filter papers ( big enough to fit in the 400 ml beaker)
- Pencil
- 2 glass rods
- 2 paper clips
- A small ruler (20 cm)
- 2 measuring cylinder with scales (50 ml3)
- scissor
- Distilled water 140 ml3
- Acetone 120 ml
- Foil sheet that Is enough to cover 2 (400 ml3) beakers.
- Black Felt-tip pens from different brands. 1 from an Expensive brand (Essentials Handwriter pen – black)
& 1 from a cheap brand (Edding colourpen board – black)
RISK ASSESSMENT- (Chromatography)
Hazard type Nature of the hazard Risk Level Control Measures PPE
Chemicals Flammable, irritant 3 Handling the liquids carefully, and if spillage occurs, report to Lab coat, and Wearing
(acetone) the lab staff and keep the area clear. Avoid sniffing the substance, plastic Gloves and googles
it can cause drowsiness. Avoid eye contact and skin contact as it
is irritant. The acetone must not be mixed with another chemical,
and any source of heat and ignition must be eliminated, such as
an open flame, as acetone is highly flammable and can cause fire
and explosions. acetone must be covered when not in use and not
exposed to air, and stored in a dry and cool area, away from heat
sources.
, / U6A3 / Chromatography experiment results with expensive and cheap felt-tip pens
Equipment Skin cuts 2 Dealing carefully with the foil. Avoid sharp ends. Wearing plastic gloves
(foil)
Equipment Skin cuts 2 Dealing carefully with scissors, placing it in a safe place. Gloves worn
(scissors)
Equipment Breakage 3 Carefully dealing with glasses and beakers, placing them in a safe Gloves are worn and
(glass) area and a case of breakage, report to the lab staff googles.
Disposal Environmental 1 After finishing, the acetone solvent will be disposed of in the Gloves worn
(acetone) sink, as a small amount of acetone will be used, and it will not
damage the environment compared to using massive amounts.
The acetone will be carefully disposed of in the sink, and all
materials been contaminated will be put away for the wash.
The method:
1. Have a chromatography paper cut in identically rectangular shapes using the scissor, that would fit into
the 400 ml3 beakers from the bottom and edges. Make sure that the papers would be straight and not curl
inside the beaker, without it touching the edges.
2. Draw a line using a pencil and a ruler roughly 1.5 cm up from the bottom of each paper.
3. Label the defined spots using the pencil on the lines of each paper; paper 1 (expensive felt-tip pen) and
paper 2 (cheap felt-tip pen) This is where we would place spots of the soluble felt-tip pens.
4. Label another line at the top of each paper within 1 cm, where we would allow the solvent to travel up
the papers before removing it from the solvent.
5. Measure 70 ml3 of distilled water using a measuring cylinder.
6. Pour the measured distilled water into one of the beakers.
7. Measure 60 ml2 of acetone using another measuring cylinder.
8. Pour the measured acetone carefully into the same beaker.
9. Repeat steps 5 - 8 for the second beaker.
10. Mark a 3 circular spot on each paper using the chosen felt-tip pens for each chromatography paper. The
spots can be medium-sized, not too small, and not too big. But the size must be identical on both papers,
make sure to leave a good distance between the spots on each paper.
11. Place a paper clip at the top of each chromatography paper. And use the glass rods to help to hook the
paper inside each beaker. So that the papers would stay still and straight during the chromatography
process. And making sure that the bottom of the paper is touching the water, but not touching the label
markings at the line.
12. Cover carefully the top of both beakers with aluminium foil sheets, without causing the papers to curl.
13. Remove the papers from the beakers just when the solvent has reached the line at the top of each paper.
14. Mark the different pigments or dyes if observed on each paper, using a pencil (label their colours if shown
on the paper)
15. Calculate and record the Rf values for each colour spot of the pigments shown on each paper.
16. Record the results on a suitable table.
17. Repeat the experiment at least 2 more times for more accurate observations.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller missmsnotes. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $23.37. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
66475 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now