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Summary Unit 6 Investigative project Assignment 2-Chromatography planning
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Unit 6 Investigative project Assignment 2-Chromatography planning , distinction received
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{Are expensive felt-tip pens different from the cheaper brands? (Chromatography)}Unit 6 assignment 2 (Produce a plan for an investigative project based on the proposal)
The timeline of this project: Gantt chart :
The weeks 1st 2nd 3rd 4th 5th 6th 7th 8th 9th 10th 11th 12th 13th 14th
0 0 1 1 2 2 2 2 0 0 1 1 1 2 2 2 0 0 0 1 1 1 2 2 2 0 0 0
6 8 3 4 0 2 7 9 4 6 1 3 8 0 5 7 1 3 8 0 5 7 2 4 9 1 6 8
S S S S S S S S
The days e e e e e e e e
O
c
O
c
O
c
O
c
O
c
O
c
O
c
O
c
N
o
N
o
N
o
N
o
N
o
N
o
N
o
N
o
N
o
D
e
D
e
D
e
p p p p p p p p t t t t t t t t v v v v v v v v v c c c
Hypothesis = = = =
Variables = = = =
Aims& objectives = = = =
Researches = = = = = = = = = = = = x x x x x x x x
Method = = = = = = = = = = = =
Equipment = = = = = = = = = = = =
Risk assessment = = = = = = = = = = = = =
Literature review = = = = = = = =
Justifications = = = =
Method Limitations = = = = =
Resources = = = = = = = = = x x x x x x x x
references = = = = = = = = = x x x x x x x x
Contingency plan = = = =
The practical x
Official write up x x x x x x x x
Lab logger = =
Gantt chart = = = = = x x x x x x x x x x x
(=)Work input (x)Not started yet
The Detailed method:
1. Have a chromatography paper cut in identically rectangular shapes using the scissor, that would fit into
the 400 ml3 beakers from the bottom and edges. Make sure that the papers would be straight and not curl
inside the beaker, without it touching the edges.
2. Draw a line using a pencil and a ruler by roughly 2 cm up from the bottom of each paper.
3. Label the defined spots using the pencil on the lines of each paper; paper 1 (expensive felt-tip pen) and
paper 2 (cheap felt-tip pen) This is where we would place spots of the soluble felt-tip pens.
4. Label another line at the top of each paper within 1 cm, where we would allow the solvent to travel up
the papers before removing it from the solvent.
5. Measure 30 ml3 of distilled water using a measuring cylinder.
6. Pour the measured distilled water into one of the beakers.
7. Measure 20 ml2 of acetone using another measuring cylinder.
8. Pour the measured acetone carefully into the same beaker.
9. Repeat steps 5 - 8 for the second beaker.
10. Mark a circular spot on each paper using the chosen felt-tip pens for each chromatography paper. The
spots can be medium-sized, not too small, and not too big. But the size must be identical on both papers.
11. Place a paper clip at the top of each chromatography paper. And use the glass rods to help to hook the
paper inside each beaker. So that the papers would stay still and straight during the chromatography
process. And making sure that the bottom of the paper is touching the water, but not touching the label
markings at the line.
12. Cover carefully the top of both beakers with aluminium foil sheets, without causing the papers to curl.
13. Allow a maximum of between 30 minutes for the chromatography process. Just when the solvent has
reached the line at the top of each paper, then remove the papers from the beakers.
14. Mark the different pigments or dyes if observed on each paper, using a pencil (label their colours if shown
on the paper)
15. Calculate and record the Rf values for each colour spot of the pigments shown on each paper.
, {Are expensive felt-tip pens different from the cheaper brands? (Chromatography)}
Unit 6 assignment 2 (Produce a plan for an investigative project based on the proposal)
16. Record the results on a suitable table.
The equipment :
- 2 beakers ( 400 ml3 )
- Chromatography papers ( big enough to fit in the 400 ml beaker)
- Pencil
- 2 glass rods
- 2 paper clips
- A small ruler (20 cm)
- 2 measuring cylinder with scales (50 ml3)
- scissor
- Distilled water 100 ml3
- Acetone 50 ml3
- Foil sheet that Is enough to cover 2 (400 ml3) beakers.
- Black Felt-tip pens from different brands. 1 from an Expensive brand (Essentials Handwriter pen
– black) & 1 from a cheap brand (Edding colourpen board – black)
The Contingency plan:
• If there was an issue with using the filter chromatography paper, or if not were available, normal
notebook papers will be used.
• If the desired felt tip pen brands that I am planning to use were not working, I will be using the
other two brands available at the laboratory.
• If all the 4 felt-tip pens are not working, I will be using pens from my own or requesting from
the other classmates if available.
• If there was an issue with distilled water, or non will be available, I will be using tap water.
• If the desired beaker size were not available, I will be using another sized beaker.
• I will use my ruler to cut the papers used if no scissors will be available.
• I will use another sizer measuring cylinder or other equipment with a scale to measure the
solvents. If I will not be able to use suitable measuring equipment and cylinders with scales, I
will use my ruler to observe the scales.
• If acetone will not be available, I will be using only distilled water as the solvent. As well as I
will not be covering the top of the beakers if the foil will not be available.
The replacements of the contingency plan will decrease the level of accuracy in this practical.
This is because the original plan and equipment required would provide precise, and valid
results. But it also does not provide as much accuracy due to limitations and equipment quality.
However, the equipment in the college lab is sufficiently reliable to observe good results within
this experiment. Hence, the contingency plan would decrease the level of reliability, as the
mentioned replacements are not as good as the original equipment needed for the
chromatography. For instance, the chromatography paper is made of material that will help
separate the pigments of the ink mixture, unlike the notebook papers, which do not have this
specific target. However, we still might observe results with the contingency plan, but the
accuracy will be reduced.
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