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Lösungsblatt zum Bioinformatikteil Orien Rousseau
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Lösungen zu den Bioinformatik Aufgaben von Orien Rousseau, KIT, 4. Semester, Allgemeine Biologie Bachelor
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Kurstag 1Gruppen Nummer 19
Name + Matr. Nr.
Name + Matr. Nr.
C1 Primersequenz incl. Schnittstelle
Sequenz primer_1 AAACCATGGTGTCCAGCGACCCGAATTTC
Sequenz primer_2 AAAGCGGCCGCAACGAACGCAACGGAAAGGC
* Tm Temperaturen bitte immer für die erste Runde der PCR angeben! Berechnung über APE
pET22_BE1 Sequenz
tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgcccta
gcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccga
tttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctt
tgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataaggg
attttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcag
gtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctg
ataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctg
tttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacag
cggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattg
acgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacgga
tggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccg
aaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacg
acgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaaca
attaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagc
cggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcag
gcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatat
actttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagtt
ttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaa
aaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatacc
aaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttacca
gtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacgg
ggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttc
ccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcct
ggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgc
cagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtatt
accgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcctgat
gcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagcc
agtatacactccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctg
ctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggc
agctgcggtaaagctcatcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcg
ttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatg
ggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggt
aaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttcca
cagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacgg
aaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgct
aaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgata
atggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgac
aggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgata
aagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcgg
tcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagct
gcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagc
tgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggtt
aacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatgg
,ttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatg
ggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggt
aaacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttcca
cagggtagccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacgg
aaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgct
aaccagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccggcgata
atggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgac
aggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgata
aagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcgg
tcgagatcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagct
gcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagc
tgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggtt
aacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatgg
cgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaa
ccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacg
cgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtc
ttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagca
atggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgac
gccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgca
gggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgc
catcgccgcttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcat
actctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctcttccgggcgctatcatgccataccgcgaaaggtttt
gcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagc
accgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaac
aagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccgg
tgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggat
aacaattcccctctagaaataattttgtttaactttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctgct
cctcgctgcccagccggcgatggccatggTGTCCAGCGACCCGAATTTCTCGCTCATAAGCTTCTTGGAGTGCCTCA
ACGAGATCGAGCACGAGTTTCTGCGAGATAAAGGGGAGAATTACCCCGTTCTCGTGCGTAAACTCCGAG
AGCTGCAACAAAAGATACCCAACGACATTGCAAACCTACCCCGCGATCCCGAAACGATCCAGCAAATTCA
CCAGACTACACACAGGATTAGAGCCGTTGCCCAGGCATTTATACGTTTCGACCAGAAGTTCGTCTCTCTGT
GTTCCGAGGTCGTTCACGGCACGAGCAAAGTCATGCAGGAGTTCAATGTCGTAAGTCCTGATGTAGGGT
GTCGAAATCTTTCGGAGGATCTTCCTGCGTATCACATGCGTAAACACTTTCTCCTTACTCTCGACAACCCTT
ATCCGACTCAAGAAGAGAAAGAAACCCTCGTTAGGTTGACCAACGAATCCACAGCAAGAGTTGGCCAGT
CCAGCGTAAATCGTCCTCCGCTCGAGGTTCATCACGTCACGCTATGGTTCATTAACGCACGCCGTCGTTCT
GGATGGTCTCATATTCTGAAGAAGTTCGCACGCGAGGATCGTTCACGGATGAAACATCTGGTACGGGCC
AAGCTGTCTTCATCAAACCAGTCTACTCCTCCTTCATCGACGTATGATTCTCTCTCAAACAATCTAGACGAC
GTTCTATCAGACAACCTCGGACGACCACTCACACCTGTTGACAAGCAGCAGTTCGAAGATGACTGGGCAA
GCATGATCAGCTGGATCAAGTATGGCGTGAAAGAAAAAGTTGGAGACTGGGTCTATGATCTCTGCGCAG
CGAGCAAGAAGACTCCGAAGCCTGGCATGCCACGTCCCGTCACCACAGTAGCGAACCGGCATCCAGCGC
GCAAAACTAAACCAGCCGCAAAACCAAAGTCAAGAACAGCGAACCCGCGAGCAAGCACAACACCTTCCA
TTGACAGCACCTTGGACAGTTCAAAGCTAGAGTCGACTCCAGAACTCAGCATGTGTTCGACAGCGGACAC
GAGCTTCAGTACATTCGGGAGCAGTCTCTCAATGTCTCACTACAATCCCTTTCAGGATGGAAACGATATTC
TACAGAGTCCGACAGTCAAAGCCAGGGGCAATAGGAAAGTCAAAGCGCTTCCGAAGCGGGCTGGAAAG
CAGCAGCCTGACGAGGTCGACAATGGTAAGATTCCTTTCCTTTGCCTTTCCGTTGCGTTCGTTgcggccgcac
tcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataa
ctagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
,C2 Primersequenz incl. Schnittstelle
primername_fwd AAACCATGGTGTCCAGCGACCCGAATTTC
primername_rev AAAGCGGCCGCATCGTTTCCATCCTGAAAGGG
* Tm Temperaturen bitte immer für die erste Runde der PCR angeben! Berechnung über APE
Nukleotid-Sequenz des verkürzten bE1-Gens
ATGTCCAGCGACCCGAATTTCTCGCTCATAAGCTTCTTGGAGTGCCTCAACGAGATCGAGCACGAGTTTCT
GCGAGATAAAGGGGAGAATTACCCCGTTCTCGTGCGTAAACTCCGAGAGCTGCAACAAAAGATACCCAA
CGACATTGCAAACCTACCCCGCGATCCCGAAACGATCCAGCAAATTCACCAGACTACACACAGGATTAGA
GCCGTTGCCCAGGCATTTATACGTTTCGACCAGAAGTTCGTCTCTCTGTGTTCCGAGGTCGTTCACGGCAC
GAGCAAAGTCATGCAGGAGTTCAATGTCGTAAGTCCTGATGTAGGGTGTCGAAATCTTTCGGAGGATCTT
CCTGCGTATCACATGCGTAAACACTTTCTCCTTACTCTCGACAACCCTTATCCGACTCAAGAAGAGAAAGA
AACCCTCGTTAGGTTGACCAACGAATCCACAGCAAGAGTTGGCCAGTCCAGCGTAAATCGTCCTCCGCTC
GAGGTTCATCACGTCACGCTATGGTTCATTAACGCACGCCGTCGTTCTGGATGGTCTCATATTCTGAAGAA
GTTCGCACGCGAGGATCGTTCACGGATGAAACATCTGGTACGGGCCAAGCTGTCTTCATCAAACCAGTCT
ACTCCTCCTTCATCGACGTATGATTCTCTCTCAAACAATCTAGACGACGTTCTATCAGACAACCTCGGACG
ACCACTCACACCTGTTGACAAGCAGCAGTTCGAAGATGACTGGGCAAGCATGATCAGCTGGATCAAGTA
TGGCGTGAAAGAAAAAGTTGGAGACTGGGTCTATGATCTCTGCGCAGCGAGCAAGAAGACTCCGAAGC
CTGGCATGCCACGTCCCGTCACCACAGTAGCGAACCGGCATCCAGCGCGCAAAACTAAACCAGCCGCAA
AACCAAAGTCAAGAACAGCGAACCCGCGAGCAAGCACAACACCTTCCATTGACAGCACCTTGGACAGTTC
AAAGCTAGAGTCGACTCCAGAACTCAGCATGTGTTCGACAGCGGACACGAGCTTCAGTACATTCGGGAG
CAGTCTCTCAATGTCTCACTACAATCCCTTTCAGGATGGAAACGAT
, C3
Original AS Alanin
Original-Sequenz: ACGCTATGGTTCATTAACGCACGCCGTCGTTCTGGATGG
Primer-Sequenz: ACGCTATGGTTCATTAACCTGCGCCGTCGTTCTGGATGG
veränderte AS Leucin
Errechnete
Schmelztemperatur 78,33°C
Tm**: Anmerkung: Die Prozentwerte wurden gerundet.
** Tm Temperatur nach der AGILENT-Vorgabe
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