Clinical Chemistry I ASCP Review Questions and Answers

Clinical Chemistry I ASCP Review Questions and Answers Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR, and near UV regions of the spectrum? A. Tungsten-filament B. Hydrogen C. Deuterium D. Mercury vapor - ANSWER A most common light source for photometry in the visible region. continuous spectrum (360-800 nm). near IR and UV. Hydrogen and Deuterium (200-375 nm) Mercury vapor- not a continuous spectrum, emitting radiation at specific wavelengths Which of the following isolates light within a narrow region of the spectrum? A. Photomultiplier tube B. Monochromator C. Photovoltaic cell D. Detector - ANSWER B Use for selecting a narrow band wavelengths from a continuous spectrum. Three kinds (prisms, filters, diffraction gratings. Which of the following is not descriptive of a photomultiplier tube? A. Emits electrons proportionally to initial light absorbed B. Must be shielded from stray light C. Cannot be used with a chopper D. Amplifies the initial signal received - ANSWER C PMT- responds to the radiant energy light it absorbs by emitting electrons in a proportional amount to the initial light absorbed. Which of the following is false about a photomultiplier tube? A. Converts radiant energy (light) to electrical energy (current) B. Amplifies the current significantly C. Has a very rapid response time D. Is composed of an iron plate and a layer of selenium - ANSWER D is part of a barrier layer cell PMT- 1. light to electricity 2. Amplification 5. Which type of photodetector employs a linear arrangement that allows it to respond to a specific wavelength resulting in complete UV/visible spectrum analysis? A. Photomultiplier tube B. Phototube C. Barrier layer cell D. Photodiode array - ANSWER D 256-2048 arranged in a linear fashion Respond to a specific wavelength that results in a continuous UV/ visible spectrum. When performing spectrophotometer quality assurance checks, what is the holmium oxide glass filter used to assess? A. Linearity B. Stray light C. Absorbance accuracy D. Wavelength accuracy - ANSWER D In spectrophotometric analysis, what is the purpose of the reagent blank? A. Correct for interfering chromogens B. Correct for lipemia C. Correct for protein D. Correct for color contribution of the reagents - ANSWER D In regard to bichromatic analysis, which of the following is false? A. Absorbance is measured at the spectral absorbance peak for a blank and the sample using the same wavelength. B. Eliminates background interferences C. Sample concentration determined from difference in two measured absorbances D. Functions as a reference blank for each sample - ANSWER A The bandpass of a spectrophotometer is 10 nm. If an instalment is set at 540 nm, the wavelengths that are permitted to impinge on the sample will be within what wavelength range? A. 530-540 nm B. 530-550 nm C. 535-545 nm D. 540-550 nm - ANSWER C 540 +/- 5nm (10nm) Which of the following formulas is an expression of the Beer-Lambert law that is routinely applied to spectrophotometric