ASCP BOC EXAM Study Guide With Correct Questions & Answers

Leukocyte reduced red blood cells are ordered for a newly diagnosed bone marrow candidate . Which way is the best way to prepare this product. - ANSWER Transfuse through a log3 leukocyte removing filter the steam autoclave method of sterilization - ANSWER uses 15 lbs of pressure for 15 minutes Factors that contribute to a PCO2 electrode requiring 60-120 seconds to reach equilibrium include the: - ANSWER diffusion characteristics of the membrane Of the following, the disease most closely associated with pale blue inclusions in granulocytes and giant platelets is: - ANSWER May Hegglin Which of the follwing factors would make an organism appear to be more resistant on a disk diffusion sustibility test? - ANSWER too many organisms in the innoculum In spectrophotometry, the device that allows for a narrow band of wavelengths is the: - ANSWER Monochromator. Leukocyte-poor red blood cells would most likely be indicated for patients with a history of - ANSWER febrile transfusion reactions. In synovial fluid, the most characteristic microscopic finding in pseudogout is - ANSWER calcium pyrophosphate dihydrate crystals An index of precision is statistically known as the: - ANSWER coefficient of variation AHG coombs control cells : - ANSWER are coated with IgG antobody Which laboratory technique is most frequently used to diagnose and follow the course of therapy of a patient with secondary syphilis - ANSWER flocculation The disease most often associated with elevations of lactate dehydrogenase isoenzymes 4 and 5 on electrophoresis is: - ANSWER liver disease The most appropriate screening test for hemoglobin F is: - ANSWER Kleihauer-Betke An enzyme deficiency associated with a moderate to severe hemolytic anemia after the patient is exposed to certain drugs and that is characterized by red cell inclusions formed by denatured hemoglobin is: - ANSWER G6P DH deficiency which one of the following gram-negative bacilli ferments glucose - ANSWER Yersinia enterocolitica Photochromogens produce pigment when: - ANSWER exposed to light for 1 hour For a patient who has suffered an acute hemolytic transfusion reaction, the primary treatment should be: - ANSWER Reverse hypotension and minimize renal damage Which of the following statements most correctly describes the utility of clinical laboratory assays for tumor markers? - ANSWER tumor markers are useful in tracking the efficacy of treatment Which of the following is the most reliable test to differentiate Neisseria lactamica from Neisseria meningidis? - ANSWER lactose degradation Which of the following may be used a a positive quality control organism for the bile esculin test. - ANSWER Enterococcus faecalis The major features by which molds are routinely identified are: - ANSWER Macroscopic characteristice and microscopic morphology hemorrhage in polycythemia vera is the result of - ANSWER abnormal platelet funtion Vibrio parahemolyticus can be isolated best from feces on: - ANSWER thiosulfate citrate bile salts (TCBS) agar Antibodies composed of IgG immunoglobulin: - ANSWER can cross the placenta from mother to fetus Antibody idiotype is dictated by the: - ANSWER variable regions of the heavy and light chains Lithium therapy is widely used on the treatment of: - ANSWER bipolar affective (manic-depressive) disorder after receiving appropriate training, the first step in using a fire extinguisher is to: - ANSWER pull the pin Tumor markers found in the circulation are most frequently measured by - ANSWER immunoassays your friend calls and asks you to access his test results. Which of the following does this violate - ANSWER HIPAA Which of the following casts most frequently appears to have a brittle consistency? - ANSWER waxy Which of the following antigens is most likely to be involved in hemolytic disease of the newborn? - ANSWER Kell A Patient with a coagulopathy was transfused with FP24 (plasma frozen within 24 hours of collection) After infusion of 15mL, the patient experienced hypotension, shock, chest pain and difficulty in breathing. The most likely cause of the reaction is: - ANSWER anti-IgA False-positive results can occur for fecal occult blood due to the ingestion of: - ANSWER horseradish A consistently and repeatedly negative IFA-ANA is: - ANSWER strong evidence against5 untreated SLE What kind of antigen-antibody reaction would be expected if soluble antigen is added to homologous antibody? - ANSWER precipitation the Philadelphia chromosome is formed by a translocation between the: - ANSWER long arm of chromosome 22 and long arm of chromosome 9 Ten units of group A platelets were transfused to a group AB patient. The pretransfusion platelet count was 12x10^3/uL and the posttransfusion count was 18x10^3/uL. From this information, the technologist would most likely conclude that the patient: a) needs group AB platelets to be effective b) clinical data does not suggest a need for platelets c) has developed antibodies to the transfused platelets d) should receive irradiated platelets - ANSWER has developed antibodies to the transfused platelets A solution contains 20 g of solute dissolved in 0.5 L of water. What is the percentage of this solution? - ANSWER 4% A technician is asked to clean out the chemical reagent storeroom and discard any reagents not used in the past 5 years. How should the technician proceed? - ANSWER consult MSDS sheets for proper disposal in a the clauss fibrinogen method, the time to clot formation in plasma is measured after the addition of: - ANSWER thrombin a centrifuge head has a diameter of 60 cm and spins at 3,000 RPM. What is the maximum achievable G force? (G=0,00001 x radius in cm x RPM) - ANSWER 2,700 G A technologist spilled 10 gallons of formaldehyde on the floor. After determining the chemical poses a significant health hazard, the first action step would be to: - ANSWER Evacuate the area A first morning sputum is received for culture of mycobacteria. It is digested and concentrated by the N-acetyl-L-cysteine alkali method. Two Lowenstein-Jensen slants are incubated in the dark at 35C with 5-10% CO2. The smears revealed acid-fast bacilli, and after 7 days no growth appears on the slants. The best explanation is: - ANSWER Cultures held for insufficient length of time. (mycobacteria are slow growers and may take up to 12 weeks) In the assay of lactate dehydrogenase, which of the following products is actually measured? - ANSWER NADH A sputum specimen from a patient with a known Klebsiella pneumonia infection is received in the lab for fungus culture. The proper procedure for handling this specimen is to: a. reject the current specimen and request a repeat culture when the bacterial organism is no longer present b. incubate culture tubes at RT in order to inhibit bacteria c. include media that have cycloheximide and chloramphenicol to inhibit bacteria and saprophytic fungi d. perform direct PAS stain; if no fungal organism seen, reject specimen - ANSWER c. include media that have cycloheximide and chloramphenicol to inhibit bacteria and saprophytic fungi Humoral antibodies are produced by which cells? - ANSWER B Lymphocytes What battery of tests is most useful in evaluating an anion gap of 22 mEq/L (22 mmol/L)? - ANSWER BUN, creatinine, salicylate and methanol Which of the following factors is used only in the extrinsic coagulation pathway? - ANSWER VII In flow cytometric analysis, low angle or forward scatter of a laser light beam provides information that pertain to a cell's: - ANSWER volume For safe operation of a centrifuge: - ANSWER ensure proper balance is maintained Which of the following activities is associated with C3b? - ANSWER opsonization Which of the following specimen requests is acceptable - ANSWER urine for culture of acid-fast bacilli In polycythemia vera, the platelet count is - ANSWER elvated A mass spectrometer detects which property of ionized molecules - ANSWER mass to charge ration Severe intravascular hemolysis is most likely caused by antibodies of which blood group system? a) ABO b) Rh c) Kell d) Duffy - ANSWER ABO urine specific gravity is an index of the ability of the kidney to: - ANSWER concentrate the urine When using the turbidity (solubility) method for detecting the presence of hemoglobin S, an incorrect interpretation may be made when there is a(n): - ANSWER increased total serum protein When cleaning up a small (5 mL) blood spill on the counter-top, the first step after donning appropriate personal protective equipment is to: - ANSWER absorb the spill with disposable absorbent material A serum sample was assayed for bilirubin at 10 AM, and the result was 12 mg/dL (205.6 µmol/L). The same sample was retested at 3 PM. The result now is 8 mg/dL (136.0 µmol/L). The most likely explanation for this discrepancy is - ANSWER the sample was exposed to light After receiving a 24-hour urine for quantitative total protein analysis, the technician must first: - ANSWER measure the total volume The causative agent of cysticercosis is: - ANSWER Taenia solium which of the following is a characteristic of polyagglutinable red cells - ANSWER are agglutinated by most adult sera The purpose of a low-dose irradiation of blood components is to: a) prevent posttransfusion purpura b) prevent GVHD c) sterilize components d) prevent noncardiogenic pulmonary edema - ANSWER prevent graft-vs-host GVHD the genus of virus associated with anogenital wart, cervical dysplasia and neoplasia is: - ANSWER papillomavirus n emphysema patient suffering from fluid accumulation in the alveolar spaces is likely to be in what metabolic state? - ANSWER respiratory acidosis An automated CK assay gives a reading that is above the limits of linearity. A dilution of the serum sample is made by adding 1 mL of serum to 9 mL of water. The instrument now reads 350 U/L. the correct report on the undiluted serum should be: - ANSWER 3500 U/L Precautions for health care workers dealing with patients or patient specimens include: - ANSWER prompt cleaning of blood spills with a disinfectant solution such as sodium hypochlorite. Which of the following is most likely to be ordered in addition to serum calcium to determine the cause of tetany? - ANSWER magnesium Aspartate amino transferase (AST) is characteristically elevated in diseases of the - ANSWER liver The appearance of normal CSF is: - ANSWER colorless and clear A blood gas sample was sent to the lab on ice, and a bubble was present in the syringe. The blood has been exposed to room air for at least 30 minutes. The following change in blood gases will occur - ANSWER PO2 increased/HCO3 decreased A technologist spilled concentrated hydrochloric acid on his/her clothing and skin, affecting a large portion of the body. After removing involved clothing, the next first aid treatment step would be to: - ANSWER use emergency safety shower and flush body with water. If present, a characteristic that is helpful in separating Pseudomonas aeruginosa from other members of Pseudomonas family is: - ANSWER growth at 42ºC A patient with uncontrolled diabetes mellitus will most likely have: - ANSWER a pale urine with a high specific gravity a blood donor has the genotype: hh, AB. What is his red blood cell phenotype? - ANSWER O Darkfield microscopy is can be used to visualize? - ANSWER Treponema palidum an organism from a peritoneal abscess is isolated on kanamycin-vancomycin laked blood agar and grows black colonies on BBE agar. It is nonpigmented, catalase positive and indole negative. The genus of this organism is: - ANSWER Bacteroides