(MB) ASCP Study Exam 2023 with 100% correct answers

What is metagenome? Sampling the genome sequence of a community of organisms. Polymorphism involved for Irinotecan? UGT1A1 NGS related to deletions and insertions, coverage, depth? -Paired-end reads for detection of indels -Coverage is how much of your target has been read -Depth is how many times a unique position has been covered Polymerase for lagging strand? Euk - DNA Pol Alpha Pro - DNA Pol I Polymerase for leading strand? Euk - DNA Pol Delta and DNA Pol Epsilon Pro - DNA Pol III Polymerase used in LAMP? -Loop-mediated Isothermal Amplification utilizes Bst Polymerase -Has helicase activity and activity at annealing/elongating temps Highest resistance gene in enterococcus? vanA has the highest resistance in enterococcus Resistance in mecA gene in MRSA codes for? mecA responsible for antibiotic resistance in S. aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam antibiotics like penicillin. Important in gene expression, defense in eukaryotes? Person guessed mRNA - gene-expression and codes for proteins that bind to DNA to promote/inhibit expression. Donor/Recipient - what's informative loci? No matching alleles is informative, you can tell D from R; non-informative would be both D&R matching. Bond between DNA and histones? Ionic - DNA is negative and histones are positive Amino Acids that make up histones? Arginine and Lysine - positively charged amino acids Multiplex modifications for low yield? Person guessed KCl. Increasing Mg2+ could increase yield. Decreasing stringency increases yield, but increases chances of mispriming PCR troubleshooting Increase Temp -- Increase Stringency Increase Probe Length -- Increase Stringency Increase Probe Complexity -- Increase Stringency Lower Salt Conc. -- Increase Stringency Remove Mg2+ -- Increase Stringency Less Time -- Increase Stringency Add Formamide -- Lowers Stringency Add Bovine Serum -- Lowers Stringency Increase Probe Conc. -- Lowers Stringency