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Samenvatting Celbiologie deel Biologie 1e bach BMW UHasselt $8.56
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Samenvatting Celbiologie deel Biologie 1e bach BMW UHasselt

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Samenvatting van de leerstof voor het vak celbiologie deel biologie zoals gegeven in academiejaar 2015/2016.

Last document update: 8 year ago

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  • October 6, 2016
  • November 19, 2016
  • 29
  • 2015/2016
  • Summary

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By: elisa_sim • 8 year ago

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ZSO 1 H7 CELSTRUCTUUR EN FUNCTIE
1. Cellen bekijken
Microscopie
Licht microscopie (LM)

Parameters zijn vergroting, resolutie en contrast. De vergroting wordt beperkt door de resolutie, deze bepaald de
helderheid van het beeld (de min. afstand tussen 2 punten die nog zichtbaar wordt waargenomen). Het contrast kan
verbeterd worden d.m.v. kleuring of labelling.
Elektronenen microscopie (EM)

Gebruikt om o.a. organellen te zien. Elektronen hebben een korte golflengte dan licht  betere resolutie. Nadeel is
dat de cel dood gaat bij het prepareren.

- Scanning elektronen microscopie (SEM): geeft 3D beeld van oppervlakte. Elektronen straal gaat door
preparaat bedekt met laagje goud.
- Transmissie elektronen microscopie (TEM): geeft 3D beeld van interne structuur. Het preparaat is gekleurd
met atomen of zware metalen  lokaal grotere elektronen dichtheid  minder elektronen doorgelaten.

Cel fractionering
1. Homogenisatie
2. Centrifugatie
3. Differentiële centrifugatie: fractionering o.b.v. massa.
Pellet bevat specifieke cel onderdelen.




2. Eukaryoot vs. Prokaryoot
Overeenkomsten
Plasma membraan, cytosol, chromosomen en
ribosomen

Verschillen
- Locatie DNA:
• EUR nucleus met dubbel membraan
• PRO nucleoid zonder membraan
- Organellen
• EU wel, PRO geen

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