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Unit 11D genetics and Genetic Engineering grade achieved D
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Unit 11D Genetics and Genetic Engineering grade achieved D
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Unit 11- Genetics and Genetic EngineeringAssignment D: Explore basic DNA techniques and the use of genetic engineering technologies
D.P6: Extract, separate and amplify DNA.
D.P7: Explain the use of genetic engineering technologies industry and medicine.
D.M6: Analyse the uses of genetic engineering technologies in industry and medicine.
D.D4: Evaluate possible future uses of genetic engineering technologies.
D.P6: Extract, separate and amplify DNA.
Title:
DNA Extraction
Introduction:
DNA is a long molecule that contains the genetic code for
all organisms. All organisms have DNA inside every one of
their cells. DNA tells an organism how to develop and
function.
Background Information/Discussion:
Each and every cell has DNA inside of it (expect mature red blood cells in humans). Each cell has an
entire copy of the same set of genetic material, which is called the genome. DNA is studied for many
reasons: such as to figure out how certain instructions stored DNA helps your body to function
properly. DNA is used to make new medicines, or genetically modify crops to make them resistant to
insects. It can also be used to solve crimes and even the use of ancient DNA to reconstruct
evolutionary history.
Equipment:
Ziplock bag
2 Strawberries (either fresh or frozen)
2tsp dish soap
½ cup water
2 plastic cups
1 coffee filter
½ cold rubbing alcohol/90% ethanol
1 stirrer or wooden stick
Method:
1) Remove the stems of the strawberry if they haven’t been removed already.
2) Put the strawberries into the Ziplock bag and smash them for about two minutes to crush
them completely. This will break open the cell walls and release the DNA.
3) In the plastic cup, make your DNA extraction solution by mixing together2 tsp dish soap, 1
tsp salt and ½ cup of water.
4) Add 2 tsp of DNA extraction liquid into the bag with the strawberries. This will further break
the cell walls.
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