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BIOL 10016 Experiment 7 Thin Layer Chromatography of Amino Acids (2023)(NEW)
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BIOL 10016 Experiment 7 Thin Layer Chromatography of Amino Acids
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MOHAWK COLLEGE OF APPLIED ARTS AND TECHNOLOGYCHEMICAL ,ENVIRONMENTAL & BIOTECHNOLOGY DEPARTMENT
Lab Report
ROOM NO: FE E309
Class : BIOL 10016 Lab.
EXPERIMENT NO : 7
TITLE : Thin Layer Chromatography of Amino Acids
Submitted by :
Partners :
Instructor : Farag Soliman
Date lab performed : February 3, 2023
Date of submission : February 10, 2023
FENNELL CAMPUS
HAMILTON, ONTARIO
1
, Purpose
The purpose of this experiment was to teach the student how to perform Thin
Layer Chromatography. This experiment will introduce the students to different
types of amino acids, reagents, and solutions that will be applied to perform the
experiment. By the end of the experiment, the students will understand the
different mobile phases for each amino acids, and calculate their Rf values for data
variations.
Procedure
Biotechnology 1 Laboratory Manual BIOL 10016 Lab. Experiment 7 Thin Layer
Chromatography of Amino Acids. Pages 2-5.
Materials
Tools
TLC Plates
TLC Chamber
Pencil
Ruler
Clips
Blower
Reagents
Alanine, Glutamic Acid, Histidine, Isoleucine, Proline, Tryptophan, Valine,
Unknown #2, Unknown #3
0.2% ninhydrin acetone
n-propanol: water (4:1 V/V)
n-butanol: glacial acetic acid: water (4:1:1 V/V)
Instruments
Micropipette
Bunsen Burner
Laboratory Oven
Data and Observation
2
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