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BIOL 10016 Biotechnology Lab Experiment 2 Gel Electrophoresis of DNA (2023)(NEW)
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BIOL 10016 Biotechnology Lab Experiment 2 Gel Electrophoresis of DNA
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MOHAWK COLLEGE OF APPLIED ARTS AND TECHNOLOGYCHEMICAL ,ENVIRONMENTAL & BIOTECHNOLOGY DEPARTMENT
Lab Report
ROOM NO: FE E309
Class : BIOL 10016 Lab.
EXPERIMENT NO : 2
TITLE : Gel Electrophoresis of DNA
Submitted by :
Partners
Instructor : Farag Soliman
Date lab performed : January 27, 2023
Date of submission : February 3 , 2023
FENNELL CAMPUS
HAMILTON, ONTARIO
1
, Purpose
The purpose of this laboratory experiment was to teach students to learn how to
properly perform Gel Electrophoresis with Agarose. This experiment will introduce
to students that different instruments and equipment needed for the procedure.
The students will also learn how Gel Electrophoresis separates DNA base on the
fragment’s sizes and how this technique help our society solving biological identity
problems.
Procedure
Biotechnology 1 Laboratory Manual BIOL 10016 Lab. Experiment 2 Gel
Electrophoresis of DNA. Pages 2-5.
Materials
Reagents
Lambda DNA HindIII digest
1X TBE electrophoresis buffer
Agarose
DyeNA safe gel dye
Tools
Electrophoresis Chamber
Gel Electrophoresis comb
Depth leveling gauge
Light Box
Gloves
Beaker
Stirring Rod
Instrument
Bio-Rad Gel Doc System
Micropipette
Laboratory Microwave
2
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