7/1/22, 9 :19 PM M5: Module 5 Exam - Requires Respondus LockDown Browser + Webcam: Essential Microbiology with Lab-2021-Martinez
Page 1 of 19 https://portagelearning.instructure.com/courses/893/quizzes/23491 ?module_item_id=135765Due No due date Points 100 Questions 29
Time Limit 120 Minutes Requires Respondus LockDown Browser
Attempt History
Attempt Time Score
LATEST Attempt 1 47 minutes 89 out of 100
Score for this quiz: 89 out of 100
Submitted Jun 30 at 3:31pm
This attempt took 47 minutes. pts Question 1
Your Answer:Define the concept of universal precautions.
Universal precautions are a standard set of guidelines to prevent the
transmission of bloodborne pathogens from exposure to blood and
other infectious materials. It means that any and every sample, whether
known or unknown, are to be treated as potentially hazardous
(pathogens) material. BIO 171 M5: Module 5 Exam - Requires RespondusLockDown Browser + Webcam 7/1/22, 9:19 PMM5: Module 5 Exam - Requires Respondus LockDown Browser + Webcam: Essential Microbiology with Lab-2021-Martinez
Page 2 of 19https://portagelearning.instructure.com/courses/893/quizzes/23491?module_item_id=135765
Universal precautions means any and all samples, whetherknown or unknown, are to be treated as potentiallyhazardous (or pathogenic) materials. ptsQuestion 2Your Answer:List at least 3 observations a researcher would be sure to note whileassessing an unknown microbial sample.Size and shapeany observable motilityGram status; Gram-positive or Gram-negative. Presence of any chemical reaction. A lab researcher would be certain to note:1. Size and shape2. Any observable motility3. Gram status (positive or negative)4. The presence of any chemical reactions5. Changes in color localized to the organism or thesurrounding media6. Capture (or draw) images of any of the characteristicsdescribed above 7/1/22, 9:19 PMM5: Module 5 Exam - Requires Respondus LockDown Browser + Webcam: Essential Microbiology with Lab-2021-Martinez
Page 3 of 19https://portagelearning.instructure.com/courses/893/quizzes/23491?module_item_id= ptsQuestion 3
Your Answer:While observing an unknown sample of limited amounts, a researchermust determine the following observations: (1) the presence of anymotility and (2) its Gram status using the same sample—the liquidsample cannot be divided. Which would you determine first and why? You must determine the presence of any motility, prior to determiningits Gram status. Motility requires a wet mount, while gram stainingrequires heat fixing the sample. If we were to start of from the Gramstain, the heat fixation process would kill the organism and not allowingus to observe its motility. The best approach is to place the liquidculture on a glass slide, to determine its motility. Then, use the sameliquid culture to perform the heat fixing and gram staining. You must determine motility before determining the Gramstatus. Motility requires a wet mount, while Gram stainingrequires heat fixing the sample. If one were to begin withthe Gram stain the heat fixation process would kill theorganism, making any observations regarding motilityimpossible. The correct approach would be to place theliquid culture on a glass slide and determine its motilitystatus. Next, the same liquid culture can be heat fixed andGram stained. ptsQuestion 4
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