Summary
Summary OCR A biology manipulating genomes
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This document provides a quick summary about everything you need to know about manipulating genomes.
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PCR (polymer chain reactions)use
↓
DNA
Amplify short lengAS of tomake copies
examples
Identifying 2
W ze
~
viruses/monitoring ISSUE FOVeUSICS detecting
spread of typing cancers and
disease gene mutations
I I
↓
taking a small sample of PNA
and amplifying to
it study
forms
From thermus
AUOW PCR to found in
L -
Phosphodiester
aquaticas
wn at high not springs - bonds
CArchaea
remp, I rare of
bacreria) TAG DNA
reaction without denaturing ~polymerase
M
components of
2
sample
DNA SOWMM binding
copied
und
site
↓ primers
nucleotides
DNA t
↓ Binds to the
forms the new end of DNA
DNA ↓
To initiate
replication
, Process 2 55° (Annealing)
0 94-96° ↓
↓ bind
primers
denaturation
↓
Hydrogen bands 3 Thi Celongation)
broken t
double,stranded
↳
polymerisation
single stranded
no, of cycles
10. of initial x 2
strands
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