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Summary OCR A biology manipulating genomes $7.10
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Summary OCR A biology manipulating genomes

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This document provides a quick summary about everything you need to know about manipulating genomes.

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  • May 18, 2023
  • 11
  • 2022/2023
  • Summary
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PCR (polymer chain reactions)
use




DNA
Amplify short lengAS of tomake copies
examples

Identifying 2
W ze
~




viruses/monitoring ISSUE FOVeUSICS detecting
spread of typing cancers and
disease gene mutations

I I

taking a small sample of PNA

and amplifying to
it study



forms
From thermus
AUOW PCR to found in
L -
Phosphodiester
aquaticas
wn at high not springs - bonds
CArchaea
remp, I rare of
bacreria) TAG DNA
reaction without denaturing ~polymerase
M

components of
2
sample
DNA SOWMM binding
copied
und
site
↓ primers

nucleotides
DNA t

↓ Binds to the

forms the new end of DNA

DNA ↓

To initiate

replication

, Process 2 55° (Annealing)
0 94-96° ↓

↓ bind
primers
denaturation


Hydrogen bands 3 Thi Celongation)
broken t


double,stranded

polymerisation


single stranded


no, of cycles
10. of initial x 2
strands

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