Summary
Summary Pharmaceutical microbiology
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Industrial pharmacy - 1st semester Includes bullet points, key diagrams and images
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Pharmaceutical MicrobiologyGroups
Eukaryotes- true nucleus with membrane
Prokaryotes- nucleus without a membrane
Bacteria
Peptidoglycan cell wall
Outer membrane of lippolysaccharides and proteins
Cytosol cytoplasm
Ribosomes are granular inclusions
Thylakoids for photosynthesis
Flagellas for movement
Fimbriae to attach to surfaces
Nucleoid circular DNA
Plasmids are accessory DNA rings
Fungi
Heterotrophic-consume food
Composed of filaments called hyphae
Chitin cell wall
Don’t have flagella
Protozoa
Heterotrophic
No cell wall
Usually motile
Specialised vacuoles for digestion and water elimination
Can reproduce through mitosis and meiosis
Viruses
No cellular structure
Replicate through host cells
Don’t metabolise or respond to stimuli
Growth of Bacteria
Measured through number of cells or cell mass
CFUs or colony forming unit
Cell number:
Direct microscopic counts- using counting chambers but dead cant be
distinguished from living
Electronic counting chambers- counts numbers and measures size distribution
Indirect viable cell counts- plating out samples so viable ones grow
Cell mass:
Direct physical measurement- dry or wet weight and volume of cell of
centrifugation
Direct chemical measurement- of chemical components such as proteins or DNA
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