BTEC Applied Science Level 3 Unit 6 Learning Aim C + D - Safely undertake the project, collecting, analysing and presenting the results and Review the investigative project using correct scientific principles
Distinction Grade Recieved
How does cost affect the effectiveness of disinfectant agains...
How does cost affect the
effectiveness of disinfectant
against E-coli?
Hypothesis
The higher the cost of the cleaner the higher the efficacy the cleaner will be.
Null Hypothesis
There is no correlation between the cost of cleaners and the effectiveness of them.
Aims and Objectives
The aim of this research is to find out if the cost of cleaners affects the effectiveness of them.
Objectives one would be to find out the best method for finding out my aim.
My second objective would be to gather a list of suitable cleaners and carry out the experiment.
My third objective is to make sure I have a quantifiable way of measuring the efficacy.
Fourth object would be to analyse the results to if my hypothesis was correct.
Equipment
Nutrient Agar Plate
Sterile Swabs
Bunsen Burner
Escherichia Coli
Distilled water
Bleach
Black Pen
Paper
Hole Punch
Tweezers
Tape
Gloves
Pipettes
Measuring Cylinder
Ethanol
Store Bought Cleaner
Method
1. Divide the petri dishes into either 2 or 3 sections (3 for bleach, 2 for cleaners and 2
for ethanol and water) and label each section with what cleaner is going to be in that
section.
2. Open sterile swab by Bunsen flame
3. Dip the swab into the E-coli, open the Agar plate and swab the E-coli onto it.
4. Measure out 1ml, 5ml and 10mls of bleach in to 3 separate measuring cylinders and
fill the 1ml and 5ml ones up with water to make 10ml.
5. Hole punch some paper and take out the small circles.
, 6. Using tweezers dip the paper circles into the different liquids and place each one on
a section of the agar plates and close the petri dishes.
7. Tape the petri dishes so the lid doesn’t come of and keep them upside down to stop
condensation dripping on them.
8. Leave for 3-7 days.
9. After growth measure the area of inhibition
Results
Cleaner Zone of Inhibition (ZoI) (mm) Price
Water 5 0
Ethanol 5.8 7.99
Branded (Zoflora) 6.7 1.08
Non-Branded (Power Force/Aldi) 7.1 0.4
Bleach 9.4 0.03
½ Bleach ½ Water 7.8 0.015
1/10 Bleach 9/10 Water 17 0.003
Mean/mx 8.4 1.359714286
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