Summary
Samenvatting Hematologie 2: laboratoriumdiagnostiek secundaire hemostase
- Course
- Institution
Een overzicht en bespreking van alle labotechnieken die gebruikt worden om een aandoening in de secundaire hemostase op te sporen.
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Hematologie 2: labdiagnostiek secundiarehemostase
Geactiveerde partiële tromboplastinetijd (aPPT)
o Algemene screeningstest intrinsieke – common pathway
o Materiaal
- Stollingsanalyzer
- Citraatplasma PPP
- Calciumionen
- Cefaline (fosfolipiden)
- Activator (silica) – geladen TC: activatie stolling
o Principe
- Citraatplasma + cefaline + activator
- Recalcificatie
- Tijd gemeten tot fibrinestolsel
- +/- 35 sec
Protrombinetijd (PT)
o Screeningstest extrinsieke – common pathway
o Opsporen deficiënties, leverfunctietest (vit K)
o Materiaal
- Stollingsanalyzer
- Citraatplasma PPP
- Calciumionen
- Tromboplastine = TF
o Principe
- Citraatplasma + TF
- Recalcificatie
- Tijd tot vorming fibrine
o INR waarde (standaardisatie) = (PT patiënt)/(PT normaal) |S| (met S = gevoeligheidsindex)
≈ 2,0 – 3,0
Mengproef
= verlengde aPTT of PT stollingsfactordeficiëntie? Stollingsinhibitor?
o Materiaal
- Stollingsanalyzer
- Citraatplasma PPP patiënt
- Citraatbloed controleplasma
o Principe
- 1:1 PPP – controleplasma
- 1u incubatie bij 37°C PT of aPPT
- Normalistaie 2u bij 37°C
Normalisatie: factordeficiëntie
Verlengd: traag werkende inhibitor
- Geen normalistaie stollingsremmer
1
Hematologie 2: labdiagnostiek secundaire hemostase
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