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Samenvatting Hematologie 2: laboratoriumdiagnostiek secundaire hemostase $3.21   Add to cart

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Samenvatting Hematologie 2: laboratoriumdiagnostiek secundaire hemostase

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Een overzicht en bespreking van alle labotechnieken die gebruikt worden om een aandoening in de secundaire hemostase op te sporen.

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  • June 17, 2023
  • 3
  • 2022/2023
  • Summary
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Hematologie 2: labdiagnostiek secundiare
hemostase
Geactiveerde partiële tromboplastinetijd (aPPT)
o Algemene screeningstest intrinsieke – common pathway
o Materiaal
- Stollingsanalyzer
- Citraatplasma PPP
- Calciumionen
- Cefaline (fosfolipiden)
- Activator (silica) – geladen TC: activatie stolling
o Principe
- Citraatplasma + cefaline + activator
- Recalcificatie
- Tijd gemeten tot fibrinestolsel
- +/- 35 sec

Protrombinetijd (PT)
o Screeningstest extrinsieke – common pathway
o Opsporen deficiënties, leverfunctietest (vit K)
o Materiaal
- Stollingsanalyzer
- Citraatplasma PPP
- Calciumionen
- Tromboplastine = TF
o Principe
- Citraatplasma + TF
- Recalcificatie
- Tijd tot vorming fibrine
o INR waarde (standaardisatie) = (PT patiënt)/(PT normaal) |S| (met S = gevoeligheidsindex)
≈ 2,0 – 3,0

Mengproef
= verlengde aPTT of PT  stollingsfactordeficiëntie? Stollingsinhibitor?

o Materiaal
- Stollingsanalyzer
- Citraatplasma PPP patiënt
- Citraatbloed controleplasma
o Principe
- 1:1 PPP – controleplasma
- 1u incubatie bij 37°C  PT of aPPT
- Normalistaie  2u bij 37°C
 Normalisatie: factordeficiëntie
 Verlengd: traag werkende inhibitor
- Geen normalistaie  stollingsremmer

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Hematologie 2: labdiagnostiek secundaire hemostase

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