APOG is a hypothetical polymorphic short tandem repeat (STR) locus in
0 view 0 purchase
Course
HBO Small Business en Ondernemerschap
Institution
HBO Small Business En Ondernemerschap
APOG is a hypothetical polymorphic short tandem repeat (STR) locus in humans with a
repeating unit of [CAGA]. The polymorphic region is shown below as a box with 25 bp of
flanking DNA sequence on each side. The n represents the number of times [CACA] on the top
strand is repeated (the polymorphi...
APOG is a hypothetical polymorphic short tandem repeat (STR) locus in humans with a
repeating unit of [CAGA]. The polymorphic region is shown below as a box with 25 bp of
flanking DNA sequence on each side. The n represents the number of times [CACA] on the top
strand is repeated (the polymorphic part of STR locus). (a) You plan to use PCR to genotype
individuals for the APOG locus. If PCR primers must be 18 nucleotides long, design a pair of
primers to amplify the APOG locus? Design the primers such that they are immediately adjacent
to the [CAGA] repeat (i.e. don\'t worry about optimizing annealing temperature, GC content, etc.
-everybody\'s primer sequences should be the same). (b) Consider APOG alleles with 9 and 7
copies of the repeating (CAGA) unit. Using the primers you have designed, what will be the
sizes in base pairs of the PCR products for each allele? In other words, if run on an
electrophoresis gel, what would be the sizes of the two bands? (c) There arc five known alleles
of APOG with 14, 11, 10.9 and 7 copies of the repeating unit. How many possible human
genotypes are there for these alleles, and what are they?
Solution
Rv primer 5’ TCACGTAACGAATGGTAC 3’
18 + 18 + 36 = 72 bp
In case of 9 copies repeating units the PCR product will be length of both the primer + 5* 4
18 + 18 + 20 = 56 bp
They will be 14-11, 14-10, 14-9, 14-7, 11-10, 11-9, 11-7, 10-9, 10-7, and 9-7
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller meejuhaszjasmynspe52866. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.32. You're not tied to anything after your purchase.