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BIOCHEM TESTS FOR BACTERIA

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An organized table for Bacteriology/Microbiology students of the different biochemical tests for bacteria including its principle, indicators, color changes and quality control.

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  • February 16, 2017
  • 2
  • 2016/2017
  • Class notes
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TEST PRINCIPLE INDICATORS COLOR QUALITY CONT
CARBOHYDRATE FERMENATION  A/A Gas+ Escheric
TEST Determines whether gram-neg rod  PHENOL RED- phenolsulfunphthalein  ACIDIC – YELLOW  K/A H2S+ Salmon
1. TRIPLE SUGAR IRON utilizes glucose, lactose, sucrose (pH indicator)  ALKALINE – RED  K/NC or K/K Pseu
fermntatively and forms H2S.  FERROUS AMMONIUM SULFATE  NEUTRAL - ORANGE aeruginosa
-H2S2 indicator
INDOLE TEST  POSITIVE –  KOVAC: (+) E. coli
Tryptophan to pyruvic acid, ammonia RED/RED/VIOLET pneumoniae
1. KOVAC’S  PDAB – add para-  NEGATIVE – YELLOW  ERLICH: (+) Elizab
dimethylaminobenzaldehyde  ORANGE – presence of meningoseptica (-
2. ERLICH’s’  PDAB add Xylene/Ether skatole/methyl indole EO 2 (Paracoccus
 INDOLE: (+) E.coli
3. SPOT INDOLE  1% PDAB Enterobacter
METHYL RED / VOGUES-PROSKAUER Determine the ability of an organism to  MPVP – (+) DEEP RED  (+) Escherichia co
TEST produce and maintain stable acid end  BUFFERED PEPTONE  (-) Enterobacter c
products from glucose fermentation  METHYL RED – large amount of acid GLUCOSE BROTH - (+)
1. MPVP MEDIUM BRIGHT RED

2. BUFFERED PEPTONE (+)… ACIDIC = PH 4.5 below
GLUCOSE BROTH (-)…. NO COLOR CHANGE = >4.5
CATALASE TEST Differentiates Staphylococcus species  (+) EFFERVESCENCE  (+) Staphylococcu
from Streptococcus species (Bubble formation)  (-) Streptococcus
 (-) Lack or weak bubble
formation
VOGUES-PROSKAUER TEST Production of Acids & Acetylmethyl  (+) PINK BURGUNDY layer  (+) Enterobacter c
carbinol (ACETOIN) from glucose  ALPHA NAPHTHOL & KOH on top of the broth in 10  (-) Escherichia col
Barrit’s method for Gram (-) fermentation mins
1. SOLUTION A (ALPHA-  (-) YELLOW
NAPHTHOL)
2. SOLUTION B (KOH)
CITRATE TEST Sodium citrate and inorganic  BROMTHYMOL BLUE  (+) BLUE – medium alkaline  (+) Klebsiella pneu
ammonium salts produce acetate and  (-) YELLOW – acid PH  (-) Escherichia col
1. SIMMON’S CITRATE SLANT carbonate  (-) GREEN – neutral PH
OXIDASE TEST Determine the ability of bacterial 1. PADAM Pink - Black  DARK PURPLE – 10  (+) Neisseria gono
cytochrome oxidase using the the -para-aminodimethyl aniline seconds  (-) Escherichia col
substrate tetramethyl para- monohydrochloride
phenylenediamine dihydrochloride to
indophenol 2. TMPDD Blue – Dark Purple
-1% tetra-methyl para-
phenylenediamine dihydrochloride

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