MT AAB Chemistry Q&A 2023

Which of the four elements in proteins differentiated this class of substances from carbohydrates and lipids? - Answer- Nitrogen Which of the proteins is soluble in water? - Answer- Albumin How are amino acids joined together to form a chainlike structure? - Answer- Peptide bonding Proteins may become denatured when subjected to mechanical agitation, heat, or chemical treatment. What does denaturation of proteins refer to? - Answer- Alteration of tertiary structure Which aminoacidopathy is NOT caused by an enzyme deficiency? - Answer- Cystinuria These aminoacidopathies are caused by an enzyme deficiency: - Answer- Maple syrup disease, phenylketonuria, and alkaptonuria How many immunoglobulin classes are currently recognized? - Answer- Five Which reagent is employed in the serum protein determination? - Answer- Biuret Why should hemolyzed serum not be used for total protein analysis? - Answer- Hemoglobin is absorbed at the same wavelength as protein in the reagent Which of the following is NOT true of the biuret reaction? - Answer- It is relatively free from interference from lipids and hemoglobin Which of the following is true of the biuret reaction? - Answer- Follows Beer's law for a reasonable range of protein, depends on the presence of at least two peptide linkages, and is insensitive to the low protein levels of urine. Why is bromcresol purple the preferred indicator for albumin dye binding techniques? - Answer- There is less interference from pigment(s) What is the most anodic protein on electrophoresis at pH 8.6? - Answer- Albumin The total protein is 7.1 g/dL. The serum protein results are: Albumin 53.0% 3.8 g/dL Alpha1 3.6% 0.3 g/dL Alpha2 11.0% 0.8 g/dL Beta 17.6% 1.2 g/dL G