ACD/CPD/CPD2 - ANSWER 21 days
CPDA-1 - ANSWER 35 days
Infants
HDFN from ABO - ANSWER Spherocytes
1st pregnancy
O mother
Permanent deferral - ANSWER Hepatitis after 11
HIV
T. Cruzi
CJD
1 unit or PRBC - ANSWER Raises hemoglobin 1g and hematocrit 3%
Leukoreduced RBCs - ANSWER Less than 5 x ...
Type 2 hypersensitivity - ANSWER Antibody depending cytotoxicity
, Transfusion reactions
Hashimotos
Good pasture
Type 3 hypersensitivity - ANSWER Immune complex
Rheumatoid arthritis
SLE
Type 4 hyper sensitivity - ANSWER Delayed
Monocytes and lymphocytes
Ouchterlony - ANSWER Antibodies added to pre-cut wells in center of agar plate and
patient Sera and standards are alternated in wells surrounding the center well
EIA/ELISA - ANSWER Sandwich technique
HCG
Nephelometry - ANSWER Insoluble complexes
Why is pass-through suspension scattered light absorbance is proportional to the
number of insoluble complexes compared to standards
Antibody concentration
Immunofluorescence direct - ANSWER Add florescence labeled anti-body to patient
tissue wash and examined under fluorescent microscope
Immunofluorescence indirect - ANSWER Add patient serum to reagent wash add
florescence label to anti-globulin wash and examined under microscope
FPIA (Fluorescence Polarization Immunoassay) - ANSWER Add reagent antibody and
fluorescent tact antigen to patient serum
Increase polarize light as a negative test decrease polarized light as a positive test
Sensitivity - ANSWER TP/ TP + FN x 100
Specificity - ANSWER TN / TN + FP x 100
Non lattice - ANSWER More sensitive immunoassays nephelometry
Lattice - ANSWER Less sensitive
C reactive protein - ANSWER Acute phase protein
Inflammation
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