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Lecture notes Unit 18 - Gene Technology
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Summary notes for unit 18 Gene technology a level caie biology
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GENE TRANSFERremovingandgene from
0 a one
organism
another
transferring it to
so that the
organism
gene
is Expressed
€
( t
isolation
gere
> -1mi
a)
restriction
restriction ☒M
④
Endonuclease
endonuclease "" """
%
> <
recombinant plasmid
t
, the desired
identification of
◦
gere
◦
be isolated
gere
can
chromosome
→
cutting from a restriction
using Endonuclease
or
using transcriptase to make
single
→
reverse a
strand
of complimentary DNA
from
another
mRNA
double synthesizes
polymerase poly nucleotide
→
DNA
to
form stranded
complimentary DNA
-
chain reactors
multiplication of the (
gere Polymerase
◦
vector
Transfer of gene into
◦
organism using
a
the cells with the
Identification of
◦
using
new
gere
genetic marker which then
a is cloned
GEI-VETICTECHNOL-OGYTOO.si
)
Enzymes restriction
→
Endonuclease
→
reverse transcriptase
ligase
→
→
DNA
polymerase
2)
synthetic DNA
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