BCH210 Final exam (CYCLES) Questions With Complete Solutions
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Course
BCH210
Institution
University Of Toronto (U Of T
)
What does Rotenone and amytal do? correct answer: Inhibit electron flow from complex I to CoQ
What does antimycin A do? correct answer: blocks complex III
What does Cyanide, azide and Co do? correct answer: Inhibit complex IV
what does oligomycin do? correct answer: inhibits ATP syntha...
BCH210 Final exam (CYCLES) Questions
With Complete Solutions
What does Rotenone and amytal do? correct answer: Inhibit
electron flow from complex I to CoQ
What does antimycin A do? correct answer: blocks complex III
What does Cyanide, azide and Co do? correct answer: Inhibit
complex IV
what does oligomycin do? correct answer: inhibits ATP
synthase, by binding to c subunit of ATP synthase blocking the
flow of protons
What are uncouplers? correct answer: disconnect ATP synthesis
from electron flow, have ability to transport protons across inner
mitochondrial membrane so ATP synthesis and electron flow are
uncoupled
Describe the property of uncouplers correct answer: - They
have hydrophobic groups that allow them to cross the membrane
= aromatic rings
- Acidic groups can bind H+ and move them from high to low
concentrations, disrupting the proton gradient and thus ATP
synthesis
Describe the structure of ATP synthase correct answer: - F1 is
the peripheral protein unit that carries out the catalytic synthesis
, of ATP in the mito matrix = consists of the stator and the rotor
shaft
- The stator holds the F1 portion in place, so it is not rotating
buts instead exhibits a confirmational change in shape
- F0 is the integral membrane protein unit that anchors the
enzyme com[lex in the inner membrane
- protons flow through the rotor causing a rotation in the ring of
c subunits of F0
- Conformational changes in the F1 beta subunits is responsible
for ATP synthesis
- There are 3 alpha and beta subunits and the gamma subunit
runs up the middle of it
Describe Boyer's binding change mechanism correct answer: -
This is how ATP is made
- beta subunit functions independently and there are 3
simultaneous reaction occurring depending on the state of the
subunit
- The flow of H+ in the rotor, rotates the ring of c subunits,
which rotates the gamma subunit which induces a
conformational change in the beta subunits
What are the 3 states the beta subunits can exist in? correct
answer: Open/ Empty => ATP released
Loose => ADP and Pi bound
Tight => ATP bound
Why does there need to be 3 states? correct answer: so a steady
stream of ATP can be made
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