100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
BCH210 TT2 Questions With Complete Solutions $13.99
Add to cart
Quickly navigate to
Preview
  2.  
  3. University Of Toronto (U Of T )
  4.  
  5. BCH210
  6.  
  7. BCH210

Exam (elaborations)

BCH210 TT2 Questions With Complete Solutions
 0 purchase
  • Course
  • BCH210
  • Institution
  • University Of Toronto (U Of T )

Functions of biological membranes correct answer: - barrier (import/export) -compartmentalization of essential molecules (incrs. efficiency) - cell signaling-mediated by pr & lipids (receptors) - modulate cell-cell recognition (glycoproteins & glycolipids) What's responsible for membrane for...

[Show more]

Preview 3 out of 26  pages

Document information

  • August 15, 2023
  • 26
  • 2023/2024
  • Exam (elaborations)
  • Questions & answers

Subjects

  • bch210 tt2 questions with complete solutions

Written for

  • University of Toronto (U of T )
  • BCH210
  • BCH210
All documents for this subject (25)

Seller

avatar-seller
Classroom

Reviews received

Content preview

BCH210 TT2 Questions With
Complete Solutions
Functions of biological membranes correct answer: - barrier
(import/export)
-compartmentalization of essential molecules (incrs. efficiency)
- cell signaling-mediated by pr & lipids (receptors)
- modulate cell-cell recognition (glycoproteins & glycolipids)

What's responsible for membrane formation correct answer:
decrease in ionic interactions w/ h2o (HYDROPHOBIC
EFFECT)
-h20X tails aggregate to hide from h2o (lots of n-c interactions)
-polar heads interact w/ h2o
Decreases membrane entropy

Membrane properties correct answer: -Selectively permeable
(small np), self repairing, barrier, flexible and continuous.
-Impermeable to polar/charged molecules
-Asymmetric, proteins have the same orientation but lipid
composition of the bilayer is different. Could be covalently
bound to sugars on EXC side
-most membranes have net diff in charge across bilayer bc of
enzymes

glycoproteins and glycolipids correct answer: -added chain of
oligosaccharides
-ALL biological membranes

,-PTM after synth
-protein folding & membrane insertion
-attachment done by transferases
-exterior of cells
-Cell-cell recognition

Flippase correct answer: (P-type ATPase) (Translocase)
-moves PE and PS from outer to cytosolic leaflet
-FLIP IN
-driven by ATP hydrolysis

Floppase correct answer: (ABC transporter) (Translocase)
-moves phospholipids from cytosolic to outer leaflet
-FLOP OUT
-driven by ATP hydrolysis

Scramblase correct answer: -translocates random phospholipids
between two monolayers randomly, resulting in an equal
distribution
-lipids in either direction
-toward eq (down concentration gradient)

membrane asymmetry correct answer: -Types of phospholipids
(PE PS inside) translocates even it out
-glycopr/lipids on outside
-ER synthesis provides proper orientation
-membrane proteins

FRAP (fluorescence recovery after photobleaching) correct
answer: Measures speed of lateral diffusion of phospholipids in
LIVE cell

, 1. Label cell surface (ex. GFP on lipids)
2. use high intensity laser to bleach small area
3. measure speed of other molecules into the region (ie. how fast
is recovery of fluorescence)
Can say how fluid the membrane is
- impacted by interactions with other components in cell or stuck
in regions like lipid rafts

1. How much light returns relative to the amount of light that
was there before the photobleaching? This is the 'percent
recovery'.
2. How fast did the fluorescent molecules migrate back into the
photobleached area? This is a measurement of the 'diffusional
mobility'.

● Application: FRAP can determine if a protein is able to move
within a membrane (high percent recovery with fast mobility),
or whether it is tethered other structural components of the cell
(low percent recovery with slow mobility).

SDS & FRAP correct answer: SDS breaks membrane--> no
FRAP

Crosslinker & FRAP correct answer: lipids link together, FRAP
is slowed

Single-molecule tracking fluorescence microscopy correct
answer: monitor movement of fluorescent molecules in
membrane
some pr & lipids can be stuck in areas for certain time ie. lipid
rafts (micro domain w/ function)

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller Classroom. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $13.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

69052 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 15 years now

Start selling
$13.99
  • (0)
Add to cart
Added