M315 Final (Possible Questions with 100% Correct Answers)

Describe the position of your hands when carrying the microscope to and from your laboratory bench. correct answers Both hands should be used-- one holding the bottom of the microscope and one holding the arm Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eye. correct answers light microscope = 0.2 um unaided human eye = 0.2 mm What two adjustments can be made to the condenser? correct answers height and diaphragm What effect does adjusting the height of the condenser have? correct answers When the condenser is raised, the amount of light on the slide is increased. What effect does opening the diaphragm have? correct answers When the diaphragm is open, the amount of light on the slide is increased. Why are condenser adjustments preferred over the use of the light intensity control? correct answers Condenser adjustments increase light without affecting the bulb light. Why is it necessary to use oil in conjunction with the oil immersion lens and not with the other objectives? correct answers The small working distance does not let enough let in alone and the oil is needed to direct more light into the lens. The oil has the same refractive index as glass. What is the relationship between the working distance of an objective lens and its magnification power? correct answers When the power of the objective lens increases, the working distance decreases. Objective lens provides that provides the highest magnification correct answers oil immersion Objective lens that provides the second-highest magnification correct answers high-dry (40x) Objective lens that provides the lowest magnification correct answers low power (5x) This objective lens has the shortest working distance correct answers oil immersion The coarse focus knob should be adjusted only when using this objective lens correct answers low power This lens collects and focuses light from the lamp onto the specimen on the slide correct answers condenser