M315 Midterm (All Solved)

Lens correct answers Ocular or eyepiece, 10x Objectives correct answers Low power 10x, high dry 40x, oil 100x Total magnification correct answers =ocular x objective Parfocal correct answers remains in focus when changing magnification Number turns for high and oil correct answers Working distance correct answers distance between objective lens and slide; decreases with increasing magnification Field of View correct answers area viewed through ocular; decreases with increasing magnification; center specimen before increasing magnification stage correct answers horizontal platform that supports the microscope slide- the stage adjustment slide is used for holding and moving the slide on the stage and the stage is adjusted by the stage adjustment knobs Light source correct answers knurled wheel for light intensity control - most microscopes have provision for reducing light intensity with a neutral density filter Condenser correct answers located under the stage that collects and directs the light from the lamp to the slide to the slide being studied - does not affect magnifing power Diaphragm correct answers within the condenser regulates the amount of light that reaches the slide Coarse adjustment and fine adjustment knobs correct answers focus Diopter adjustment ring correct answers turning curled knob without touching the focus knobs resolving power correct answers the limit for most light microscopes is 1000x which is set by an intrinsic property of lenses d=0.5*wavelength/NA(numerical aperture) limit of resolution correct answers distance between two objects Numerical aperture correct answers mathematical expression that describes how the condenser lens concentrates and focuses light rays from the light source