Uni 6 Assignment Three: Investigative Project Execution, Evaluation, and Reporting
Learning Aim: The primary objective of Uni 6 Assignment Three is to guide students in safely executing an investigative project based on the developed plan, including collecting, analyzing, and presenting results....
Unit 6 : investigation project
Project implementation and review
Fatima Kalaf
ID: 4018003
,Proposal
The purpose of this experiment is to discuss and determine the effects of different
concentrations of methanol on heart rate using flea’s daphnia. A typical light microscope
makes Daphnia plainly visible at low magnification. The heart rate, which can reach 300
beats per minute, can be measured, and quantified under a variety of circumstances, such as
changing the water's temperature, or the type and quantity of chemicals added. The
strategy offers an intriguing method for examining how various compounds affect an
animal's metabolic process. Daphnia is a poikilothermic organism, which implies that the
environment's temperature has a direct impact on both its body temperature and metabolic
rate. The rate at which the heart beats change in response to changes in metabolic rate
(cardiac frequency). methanol beverages cause Daphnia's heart rate to decrease. The heart
rate of Daphnia lowers with an increase in alcohol concentration.
Hypothesis
As the concentration of methanol increases, the heart rate decreases
Before I attempted to do my own method and experiment I did a lot of research online, I
found few different methods and secondary data on how methanol effect the heart rate of
daphnia and here is three refences I found and I’m going to talk about and discuss each one
individually.
References
1. Journal of Undergraduate Biology Laboratory Investigations
file:///C:/Users/mahdi/Downloads/8792-37914-1-PB.pdf
Hypothesis: methanol decreases heart rate of daphnia compared to water
Method and materials
They used a solution of methanol and aquarium water as well as a solution made entirely of
aquarium water to investigate the impact of methanol on the heart rate of daphnia. Using a
clicker to measure the daphnia's heart rate, we videotaped it for 15 seconds while it was
being observed under a microscope. This figure was multiplied by four to determine the
heart rate in beats per minute, or bpm. Once the daphnia was immersed in the aquarium
water solution on the slide, they used a dropper to add one drop of 5% methanol alcohol. To
give the methanol time to have an impact on the daphnia, it was submerged in the water/
methanol mixture for seven minutes. The daphnia was re-examined under the microscope
after the seven-minute period was up, and the heart rate was computed in the same
manner as it had been before the methanol was injected. To precisely gauge the rate of
change, heart rate was measured before and after the addition of the methanol. To improve
the accuracy and size of our experimental group, they repeated this with five daphnia. As
their defence, then repeated the entire procedure, with one exception: a drop of aquarium
water was added in place of the drop of methanol. This sham control was utilised to increase
, the precision of the data by demonstrating whether the heart rate was influenced by
methanol or whether it was just a result of the time spent in a slide, which undoubtedly
places some stress on the body. They then calculated the percentage rate of change in each
of the ten daphnia's first and second observed heart rates. For easier viewing, the average of
these percentages was then calculated and plotted as a bar graph. Microsoft Excel
programme was used for this. their analysis uses an unpaired T-test on the % change values
because their data were both nominal and measurement, and they had two unpaired
groups.
Results and discussion
figure 1. shows the average percent change decreasing
rapidly in Daphnia’s heart rate in 5% methanol
compared to steady average percent change in
Daphnia’s heart rate in aquarium water
The average heart rate of daphnia is much lower after immersion in 5% methanol alcohol
than it is after immersion in aquarium water, as predicted by the hypothesis (as seen on
figure 1). In the experiment, the association held true across all five daphnia. The daphnia in
their control group, which was not immersed in methanol, showed no discernible rise in
heart rate either before or after spending seven minutes on the slide. their finding that
methanol consumption reduces action potentials in myogenic organisms is supported by the
Fisher study. Given that both humans and daphnia are myogenic, it follows that methanol
lowers action potentials and, consequently, heart rate in humans. Another interpretation
might be that the lab environment has more of an impact on the heart rate reduction. The
cause of the reduced action potentials in daphnia caused by methanol consumption is
uncertain; it may be related to a reduction in acetylcholine release, a reduction in
acetylcholine receptor sensitivity, or a combination of the two. To pinpoint the precise
source of the reduction in action potentials brought on by methanol alcohol, a future
experiment may evaluate the amount of acetylcholine produced in the synapses. This could
assist in demonstrating the precise kind of heart issues that methanol could treat.
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