Answers of all the exam questions from the course 'Genome Technology and Applications' (19/20)
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Course
Genome technology and applications (2051FBDBMW)
Institution
Universiteit Antwerpen (UA)
Answers of all the exam questions from the course 'Genome Technology and Applications' (19/20): It contains a compact summary and elaboration of all the exam questions from the course 'Genome Technology and Applications' , This document can be learned in 2-3 days (19/20).
1. Give the differences and similarities of array-CGH and SNP array (max. 1 page).
2. During evolution, genes undergo many duplications. Give the different outcomes of
duplications in genes (max 1 page).
3. (a) You want to clone a PCR product into a vector. This PCR product can only be
inserted at EcoRI sites, but your PCR product doesn’t have any EcoRI sites. How
can you solve this problem (max. 10 lines)? (b) Make a table of the most common
used vectors, describe their applications in 1 line and give their insert sizes (max 1
page).
4. Explain Nanopore sequencing or PacBio sequecing.
Oral question
1. You discovered a new bird species of which the sequence is not yet present in the
database. Which sequencing technology or technologies would you use and which
ones are less suited?
Gene and Genome (With explanations from Van Camp)
The course is presented by Prof. Van Camp, Prof. Van Hul and Prof. Kooy The style leans in to
genetics from 3rd Ba. It taught using slides is based on a book for every professional, differs a bit if
you have additional needs to learn from the book.
Oral: (this is only for Prof Van Camp, appr. 2 oral questions)
1. Genetically defective search dwarfism, mental retardation, facial deformities
2. How can you avoid a smear?
3. You can find a number of patients with an autosomal recessive disease a variety of
homozygous and heterozygous frameshift mutations compound. What is probably the
disease mechanism, and which strategy would follow in order to construct an in vitro and in
vivo model for the study of gene function.
4. You are doing your master thesis in a genetic lab. You have to develop a PCR reaction point
for a particular gene fragment. However, you get a large number of bands instead of one
beautiful band on your gel. How could this be? What can you do?
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