Summary All exercises/self study assigments and solutions from Genome Technology and Applications (19/20)

self study assignment More recent and
convenient cloning systems Genome
Technology and Applications


geschreven door

Bi0med




www.stuvia.com


Gedownload door: InnePatteet |
Dit document is auteursrechtelijk beschermd, het verspreiden van dit document is strafbaar.

, Stuvia - Koop en Verkoop de Beste Samenvattingen




Self study assignment: More recent and convenient cloning systems



Prof. Van Camp

Fundamentals of gene and genome technology

Gene and genome technology




Learning aids:

Several video’s and texts on Gateway cloning and Gibson assembly are available on Blackboard



Aims:

1) Gateway cloning
 The student can describe the CcdA/CcdB selection system in cloning with technical details
 The student can describe the Gateway cloning system with technical details and required
components
 The student can describe the advantages and disadvantages of the Gateway cloning system
 The student can list and explain the differences between Gateway cloning and traditional
cloning using restriction enzymes and ligase
 The student can design a cloning experiment using the Gateway system (the student knows
which att sites to use in which stage of the cloning experiment)

2) Gibson assembly
 The student can describe the Gibson assembly with technical details and required compenents
 The student can describe the advantages and disadvantage of Gibson assembly
 The student can incorporate Gibson assembly in the theoretical design of cloning
experiments to replace restriction enzyme/ligation steps




Gedownload door: InnePatteet |
Dit document is auteursrechtelijk beschermd, het verspreiden van dit document is strafbaar.