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Summary All exercises/self study assigments and solutions from Genome Technology and Applications (19/20) $8.03   Add to cart

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Summary All exercises/self study assigments and solutions from Genome Technology and Applications (19/20)

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All exercises/self study assigments and solutions from Genome Technology and Applications (19/20): This document includes all questions AND FULL answers to the exercises of the exercise session of Genome Technology and Applications. It has the answers given by professor Guy van Camp and is thus ver...

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  • September 20, 2023
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  • 2022/2023
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Self study assignment: More recent and convenient cloning systems



Prof. Van Camp

Fundamentals of gene and genome technology

Gene and genome technology




Learning aids:

Several video’s and texts on Gateway cloning and Gibson assembly are available on Blackboard



Aims:

1) Gateway cloning
• The student can describe the CcdA/CcdB selection system in cloning with technical details
• The student can describe the Gateway cloning system with technical details and required
components
• The student can describe the advantages and disadvantages of the Gateway cloning system
• The student can list and explain the differences between Gateway cloning and traditional
cloning using restriction enzymes and ligase
• The student can design a cloning experiment using the Gateway system (the student knows
which att sites to use in which stage of the cloning experiment)

2) Gibson assembly
• The student can describe the Gibson assembly with technical details and required compenents
• The student can describe the advantages and disadvantage of Gibson assembly
• The student can incorporate Gibson assembly in the theoretical design of cloning experiments
to replace restriction enzyme/ligation steps

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