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Full respiration notes including practical and ms answers

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full respiration notes for A2 / A level AQA biology. includes answers from the mark schemes includes notes for practical for respiration

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  • October 21, 2023
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  • 2023/2024
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Respiration
Respiration occurs in animals and plant cells. For example yeast -> RP9

The main organelle it takes place in is the mitochondria


Glucose + Oxygen → Carbon dioxide + water + ATP
C6H12O6 + 6O2 → 6CO2 + 6H2O
Oxidation and Reduction

Oxidation means the release of a hydrogen molecule that then goes onto reduce another molecule

 An example is TP is oxidised which releases the Hydrogen ion from TP. This hydrogen ion is
used to reduce NAD to form NADH

Reduction means the addition of a hydrogen ion to a molecule

Mitochondria




ATP synthase




Cristae
Intermembrane
space




Aerobic respiration
Stages of aerobic respiration

, 1. Glycolysis
2. Link reaction
3. Krebs cycle
4. Oxidative phosphorylation

Glycolysis – split glucose into 2 pyruvate
 Takes place in the cytoplasm
 Glucose (6C) is phosphorylated to become phosphorylated glucose (6C)
o 2 ATP is hydrolysed to produce 2 ADP and 2 Pi (phosphate molecules)
o Reaction is endothermic – ATP is used up
 Phosphorylated glucose is phosphorylated and splits into 2 Triose Phosphate (3C) (TP)
o 2 ATP is hydrolysed to produce 2 ADP and 2 Pi (phosphate molecules)
o Reaction is endothermic – ATP is used up
 TP is oxidised into 2 pyruvate (3C)
o Oxidation of TP causes the release of Hydrogen which is used to reduce NAD to
NADH
o 2 NAD is reduced to NADH
o 4 ATP is made
 Net gain of ATP is 2

Outcome
 + 2 ATP (net gain)
 + 2 NADH
 + 2 pyruvate (3c)

Link reaction – convert pyruvate to acetyl CoA
 Takes place in the matrix
 Pyruvate passes through the mitochondria by active transport
 Pyruvate is oxidised into acetate
o NAD is reduced to NADH
o Removal of 1 CO2
 Acetate combines with co-enzyme A to produce Acetyl CoA (2C)

Outcome
 - 1 CO2
 + 1 NADH
 Acetyl CoA (2C)
 NO ATP made

Krebs cycle – reduce co-enzyme NAD and FAD
 Takes place in the matrix
 Acetyl CoA (2C) reacts with a 4C molecule (oxaloacetate)
 Co-enzyme A is released
 This forms citrate (6C)
 Citrate enters the krebs cycle where a series of oxidation and reduction reactions happen

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