100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
biochemistry - centrifugation $7.11
Add to cart
Quickly navigate to
Preview
  2.  
  3. University Of Brighton (UoB)

Class notes

biochemistry - centrifugation
 3 views  0 purchase
  • Course
  • Institution
  • University Of Brighton (UoB)

content: 1) centrifugation of blood 2) centrifugation 3)Revolution per minute (RPM) vs Relative centrifugal field (RCF) 4) Relative centrifugal field 5) Rotor types 6) Types of centrifuges 7)Types of centrifugation 8) Sucrose density gradient centrifugation to isolate polysome 9) Isopy...

[Show more]

Preview 2 out of 7  pages

Document information

  • October 28, 2023
  • 7
  • 2023/2024
  • Class notes
  • Nigel
  • All classes

Subjects

  • centrifugation
  • types of centrifuges
  • types of centrifugation
  • revolution per minute
  • relative centrifugal field

Written for

  • University of Brighton (UoB)
  • Unknown
  • Unknown

Seller

avatar-seller
ritheshaa

Content preview

Centrifugation

Centrifugation of blood
 A tube of blood will separate if given enough time.
 Centrifugation will separate most particles into fractions in a short amount of time
 The particles’ settling velocity in centrifugation is a function of their:
- Applied force
- Density difference between the particle and the liquid
- Size and shape of the molecule’s centrifugal acceleration
- Viscosity of the medium




Centrifugation
 Separates (or concentrates) particles in a liquid medium based on differences in size,
shape and density.
 Particles will sediment in a liquid of appropriate density if left long enough. Different
particles will sediment at different rates.
 Centrifugation uses centrifugal force to speed the sedimentation process up

, Revolutions per minute (RPM) vs Relative Centrifugal Field (RCF)
 RPM: how fast the centrifuge goes
 RCF: the amount of acceleration or force exerted on the sample in a centrifuge, i.e., a
function of the rotational speed of the rotor and the radius of the rotation.




Relative Centrifugal Field
 RCF is expressed as a multiple of the earth’s gravitational field (g=9.807 ms-2).
 The ratio of the weight of the particle in the centrifugal field to the weight of the
same particle when acted on by gravity alone and is then referred to as the relative
centrifugal field.
Formula: RCF=1.12x R x (speed in rpm/1000)2
R: radius in mm and rpm: revolutions per minute

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller ritheshaa. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $7.11. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

53249 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$7.11
  • (0)
Add to cart
Added