Write in soft pencil.
Do not use staples, paper clips, glue or correction fluid.
Write your name, Centre number and candidate number on the Answer Sheet in the spaces provided unless
this has been done for you.
DO NOT WRITE IN ANY BARCODES.
There are forty questions on this paper. Answer all questions. For each question there are four possible
answers A, B, C and D.
Choose the one you consider correct and record your choice in soft pencil on the separate Answer Sheet.
Read the instructions on the Answer Sheet very carefully.
Each correct answer will score one mark. A mark will not be deducted for a wrong answer.
Any rough working should be done in this booklet.
Electronic calculators may be used.
1 Which statements about resolution and magnification are correct?
resolution magnification
A the ability to distinguish between two the number of times larger an image is
separate objects that are very close compared with the real size of the object
together
B the clarity of the image formed the power of the microscope to focus
by the microscope on very small objects
C the number of times larger an image is the ability to distinguish between two
compared with the real size of the object separate objects that are very close
together
D the power of the microscope to focus the clarity of the image formed
on very small objects by the microscope
2 An eyepiece graticule has a scale with 100 divisions. A stage micrometer has a scale with
50 divisions, each of which is 0.040 mm apart.
Using a ×40 objective lens, the whole length of this stage micrometer scale lines up with
15 divisions of the eyepiece graticule.
so -o
-
of
What is the actual length of the 100 division scale of the eyepiece graticule?
A 1.3 mm B 13 mm C 75 µm D 750 µm
3 A prokaryotic cell which is 1 µm in diameter, is magnified 50 000 times in an electron micrograph.
What is the diameter of the cell in the electron micrograph?
4 A scientist carried out an experiment to separate cell structures in animal cells.
The cells were broken open to release the cell structures.
This extract was filtered into a centrifuge tube and then spun in a centrifuge. The heaviest cell
structure sank to the bottom forming pellet 1, as shown in the diagram.
liquid above
pellet
pellet 1
The liquid above pellet 1 was poured into a clean centrifuge tube and spun in the centrifuge at a
higher speed to separate the next heaviest cell structure. This cell structure sank to the bottom,
forming pellet 2.
This procedure was repeated twice more to obtain pellet 3 and pellet 4, each containing a single
type of cell structure.
Which row shows the order in which the cell structures were collected?
pellet 1 pellet 2 pellet 3 pellet 4
A nucleus lysosomes mitochondria ribosomes
B nucleus mitochondria lysosomes ribosomes
C ribosomes lysosomes mitochondria nucleus
D ribosomes mitochondria lysosomes nucleus
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