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Behaviour of biomolecules Lecture notes: Spectroscopy (Fluorescence)
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BSc Biomedical sciences course at the University of Southampton Behaviour of biomolecules Lecture notes: Spectroscopy (Fluorescence)
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BIOL1021:Behavior of Biomolecules:
Spectroscopy
Dr Philip T.F. Williamson
P.T.Williamson@soton.ac.uk
Centre for Biological Sciences
, ROYAL SOCIETY OF CHEMISTRY DATA BOOK
THE ELECTROMAGNETIC SPECTRUM
The electromagnetic spectrum
f / Hz /m
1020 3 x 1012
1019
X rays
X-ray 1018
420 nm (4.20 x 107 m)
Spectroscopy 1017 3x 109
Violet
Far ultraviolet Blue 470 nm
1016
UV/Vis
1015 3 x 107 Ultraviolet Green 530 nm
Spectroscopy
1014 Yellow 580 nm
Rotational/Vibrational Infrared
Spectroscopy 1013 3 x 105 Orange 620 nm
Rotational Far infrared
1012
Spectroscopy Red
700 nm (7.00 x 107 m)
1011 3 x 103
Electron Resonance Microwave
1010 3 x 102
(NMR/MRI)
109 3 x 101
108 3
Nuclear Magnetic
Resonance 107 Radio
(NMR/MRI)
106 3 x 102
105 2
, Interaction of electromagnetic
radiation with molecules
Resonates with
transition dipole moment (TDM)
Generates oscillating
electromagnetic
field/wave Field/Wave attenuated
H
N
N H2
OH
O
Oscillating
dipole TDM starts to oscillate (gains energy), absorbing energy for
oscillating field/wave
3
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