Report of one of the instrumental analysis experiments. Rated with a passing grade. Do not copy the name and date due to plagiarism.
Qualitative determination of a pharmaceutical formulation
based on High-performance liquid chromatography and thin
layer chromatography.
Qualitative determination of a pharmaceutical formulation
based on High-performance liquid chromatography and thin
layer chromatography.
Tymo Zwakenberg S4326253; Aniek barbier; October 10, 2022
Aim and principle
Two different methods of chromatography being high-performance liquid chromatography (HPLC)
and thin layer chromatography (TLC) are suitable for the qualitative determination of a
pharmaceutical formulation. TLC is an example of planar chromatography where the stationary
phase is located in a plane. The mobile phase then moves upward because of the capillary forces. In
order to make sure this procedure succeeds, the dried plate should be placed vertically in a thin
layer of mobile phase (eluent). The compounds of interest have been added on the plate before it’s
placed within the eluent. HPLC on the other hand has its stationary phase located inside a tube or
capillary, which is called the column. For HPLC an external force is always needed to be present to
carry the eluent along the stationary phase. A schematic overview of both setups can be found in
figure 1 and 2 [6].
Figure 1 & 2: Schematic overview of HPLC and TLC setup [4 & 5].
In order to acquire more knowledge and experimental skills with HPLC and TLC, a qualitative
determination of a given compound will be executed. This will be done by determining the effect of
methanol, pH and TEA presence on retention, executing and obtaining chromatographs and by
executing a TLC run.
, Materials and methods
All HPLC measurements were executed and recorded as chromatographs with a manual Hitachi
Primaide 1000. Whereas a TLC plate and UV-lamp were used to execute and review the TLC results.
The analyzed pharmaceutical formulation was provided as a tablet with reference number 2. The
qualitative characteristics of this tablet will be determined. The setup for this TLC experiment can be
found in the lab manual at page 41. The TA holds the list which tells which pharmaceutical
formulation corresponds with which diuretic. For all the executed steps the lab manual and/or lab
journal can be constructed. However, a few deviations on the lab manual will be listed, alongside the
materials, chemicals and calculation methods used.
Deviations:
- 10 µl was injected into the injection valve instead of 30 µl for every analysed sample.
- The drug formulation preparation was diluted a 100x before it was injected and analyzed.
- Every participant in the experiment was responsible for 2 HPLC setups, instead of 4.
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller tymozwakenberg. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $5.89. You're not tied to anything after your purchase.
