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UNE MEDICAL BIOCHEMISTRY MIDTERM TEST QUESTIONS AND ANSWERS
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UNE MEDICAL BIOCHEMISTRYMIDTERM TEST QUESTIONS AND
ANSWERS
Which of the following proteins is likely to have quaternary structure? - answer A multimeric
protein that contains multiple peptide chains
Prosthetic groups are complex nonprotein molecules that participate in catalysis by providing
functional groups that form a covalent intermediate between the enzyme and the substrate.
Which of the following is an example of a coenzyme or cofactor that participates in a covalent
catalysis reaction? - answer Pyridoxal phosphate
Which of the following best describes a protein domain? - answer A section of a protein
structure sufficient to perform a particular chemical or physical task
Cleavage of fructose 1,6 bisphosphate to dihydroxyacetone and Glyceraldehyde 3-phosphate
involves the breaking of a carbon-carbon bond through a process other than oxidation or
hydrolysis. This is achieved by what class of enzymes? - answer Lyase
A 55-year old man is brought to the emergency room with a chief complaint of chest pain and
tingling in his left arm. Elevation of which of the following enzymes in circulation would be most
indicative of myocardial infarction? - answer Troponin
Allosteric activators of hemoglobin will increase substrate binding through which of the
following mechanisms? - answer Binding the protein, and keeping it in the "R" conformation
Which of the following best describes tertiary structure? - answer Interactions between alpha
helices and beta sheets to form a domain
How do most enzymes reduce the activation energy needed to move a reaction forward? -
answer providing an active site most complementary to the transition state
, Domains are common structural elements that retain a particular function within the protein.
Which of the following is an example of a protein domain? - answer An arrangement of beta-
strands connected by alpha-helices (beta-alpha-beta-alpha)
Which amino acid would not be commonly found in the middle of an alpha-helical domain? -
answer Proline
Which of the following is a true statement with regards to the malate-aspartate shuttle system?
- answer Generates 2.5 molecules of ATP in mitochondria per each NADH from glycolysis.
Complex II (Succinate Q-reductase) is not considered part of the process of oxidative
phosphorylation because it is unable to do which of the following activities? - answer Pump
protons from the mitochondrial matrix into the inter membrane space.
Succinate is a substrate for which complex in the electron transport chain? - answer Complex II
(Succinate-Q reductase)
The glycerophosphate shuttle is the major shuttle of most tissues. Which of the following best
describes the transfer of electrons by this shuttle? - answer Electrons are directly transferred
from glycerol 3-phosphate to an FAD containing glycerol phosphate dehydrogenase.
A 19-year old male is being evaluated for muscle weakness. Evaluation of a muscle biopsy
revealed normal rates of succinate oxidation but very low rates of pyruvate oxidation. Further
testing determined normal levels of enzymatic activity in both malate dehydrogenase and
pyruvate dehydrogenase. Based on this data, the patient may have a mutation in a gene
encoding for which mitochondrial protein? - answer Complex I (NADH Q-oxidoreductase)
Which of the following components of the electron transport chain passes electrons to O2
causing it to be reduced to H2O? - answer Cytochrome c oxidase
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