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MIBO EXAM 2 PRACTICE (PT. 1) WITH COMPLETE SOLUTIONS
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MIBO EXAM 2 PRACTICE (PT. 1) WITHCOMPLETE SOLUTIONS
__________ use an internal RNA primer so that the end of the eukaryotic chromosome can be
replicated.
a. Primases
b. RNA polymerases
c. Telomerases
d. DNA polymerases
e. Helicases - answer c. Telomerases
A transmembrane sensor kinase protein senses an environmental condition outside
__________ bacteria or in the periplasm of a/an __________ bacteria.
a. Gram-positive; Gram-negative
b. Gram-negative; Gram-positive
c. prokaryotic; eukaryotic
d. prokaryotic; archaean
e. Gram-positive; acid-fast - answer a. Gram-positive; Gram-negative
In a gel shift assay, a DNA binding protein is mixed with radioactively labelled oligos of a specific
sequence. If the protein binds to the DNA sequence, the mobility of the DNA shifts, as
measured by gel electrophoresis followed by detection of radiolabel. A critical control in these
assays is the addition of cold (non-radioactive) oligos of the same sequence. As increasing
amounts of cold oligo are added, what would you expect to happen in this control?
a. An intermediate sized band will for - answer d. As more cold oligo is added, the DNA will shift
back down to the unbound size.
,Which of the following is NOT a palindrome? (Ignore punctuation).
a. a man, a plan, a canal, panama.
b. never odd or even.
c. May a moody baby doom a yam?
d. Madam, in Eden I'm Adam.
e. Murder for a jar of red jam. - answer e. Murder for a jar of red jam.
Based on what you know about regulation, which would be more likely to be ubiquinated?
a. An RNA polymerase subunit.
b. An activator.
c. A histone.
d. A cytoskeleton protein.
e. All are equally likely. - answer b. An activator.
Which molecule is responsible for removing torsional stress during DNA replication?
a. DNA primase
b. single-stranded binding protein
c. RNase H
d. DNA gyrase
e. DNA ligase - answer d. DNA gyrase
The process of plasmid addiction is based on genes that code for:
, a. short-lived toxin and short-lived antidote
b. long-lived toxin and long-lived antidote
c. long-lived toxin and short-lived antidote
d. short-lived toxin and long-lived antidote
e. high plasmid copy number - answer c. long-lived toxin and short-lived antidote
F factor contains how many origins of replication?
a. one
b. two
c. three
d. four
e. none - answer b. two
Newly replicated, and consequently __________, DNA sequences are invisible to a restriction
enzyme.
a. mutated
b. restricted
c. modified
d. hemimethylated
e. single-stranded - answer d. hemimethylated
The ________ is not transcribed and the _________ is not translated.
a. template strand; coding strand.
b. start codon; stop codon.
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