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MIBO 3500 EXAM 2 REVIEW QUESTION AND ANSWERS.
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MIBO 3500 EXAM 2 REVIEW QUESTIONAND ANSWERS
True or False (circle one): The anti-parallel nature of nucleic acids is illustrated in the replication
and expression of DNA, and even the translation of mRNA into protein. - answer True
Accumulation of the protein _____1.______ during cell growth triggers initiation of DNA
replication when it binds to the chromosome near the ________2._______ - answer 1. DnaA
2. oriR/ origin of replication
Negative regulation by ________________ results when a signaling molecule binds to a
repressor protein, which enables the repressor protein to bind to the operator region of a gene
and block its transcription. - answer repression/ corepression
Negative regulation by __________ results when a signaling molecule binds to a repressor
protein and prevents it from binding to the operator region of a gene. (2) - answer induction/
derepression
The enzyme β-galactosidase converts the disaccharide ____1._____ into the monosaccharides
____2.____ and ____3.______ - answer 1. lactose
2. glucose
3. galactose
_________ refers to the inhibition of transcriptional activation of genes for alternative carbon
and energy sources as long as the preferred substrate glucose is available. (2) - answer
catabolite repression
An E. coli strain has a mutation in the lac repressor protein so that it binds to the operator even
in the presence of allolactose. When this mutant is cultured in the presence of both lactose and
glucose, will it still exhibit diauxic growth? (2) - answer NO
, Many of the stages in forespore formation are regulated by what mechanism? - answer
alternative sigma factors
Nitrogen fixation is the conversion of N2 into - answer ammonia/ammonium
_____?_______ occurs when fertilizer runoff triggers explosive growth followed by
decomposition of phytoplankton and algae. - answer Eutrophication / Hypoxia
_____?______ is a regulatory mechanism where stalling in the translation of a protein affects
the transcription of the gene for that protein. - answer attenuation
______?______ can regulate translation by binding to the ribosome- binding site on mRNA,
resulting in the breakdown of the mRNA. - answer sRNA (small) / asRNA (antisense
____?_____ Is the ability of an organism to modify motility in response to a chemical gradient. -
answer chemotaxis
True or False (circle one): In the absence of an attractant, runs are shorter and tumbles more
frequent. (2) - answer true
The autophosphorylation of _____ is inhibited when attractant is bound to MCP's, which results
in counterclockwise rotation of the flagella and a bacterial run. (2) - answer CheA
____?____ interacts with the flagella motor to promote a clockwise rotation and tumbling. (2) -
answer CheY-P
DNA sequence analysis of marine viruses revealed (surprisingly) that many viruses carried
certain
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