Lab 8 Quiz – Microbiology
Question 1
The word ‘antigen’ is actually a combination for what two words?
Your Answer:
Antibody pathogen
Antibody-generating
Question 2
For the ELISA assay depicted below, which step (A-E) contains only specifically bound
antigens (aka a 'pure' population)?
Your Answer:
In step C you see only specifically bound antigens, aka a pure population. In step B you
see specific and non-specific antigens, but then the wash step removes the non-specific
or unbound antigens, leaving only the specific antigen which is present in step C.
Specifically bound antigens are present in step C. Specific and non-specific
antigens are present in B but then the wash step removes the non-specific
(unbound) antigens, leaving only the specific population seen in step C.
Question 3
What would happen in terms of the colorimetric readout if you forgot to do the wash step
after Step D (above)?
Your Answer:
If you forgot to do the was step after Step D, all the wells would have a dark color. This is
because the intensity of the readout is dependent upon the amount of the labeled
secondary antibody present in the well. Since the unbound secondary antibody was not
washed away, all the wells would react the same.
All of the wells would have a dark color. The intensity of the readout is
dependent upon the amount of the labeled secondary antibody present in the
well. Since the unbound secondary antibody was not washed away, all wells
would react equally.
Question 4
In a western blot ____________ are separated based on its size.
Your Answer:
Proteins
proteins
Question 5
When setting up a western blot, what is the purpose of the blocking step post-transfer?
This study source was downloaded by 100000801626315 from CourseHero.com on 09-07-2022 23:22:50 GMT -05:00
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller LATESTSOLUTION. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.99. You're not tied to anything after your purchase.
