Samenvatting in puntjes van hoofdstuk 1 (Techniek) van cytologie (cel 2). De documenten die hierop volgen zijn:
- Cytologie: Hoofdstuk 2 (De cel-veel kostbaar goeds in een fragiele verpakking)
- Cytologie: Hoofdstuk 3 (Bloed)
- Cytologie: Hoofdstuk 4 (De nucleus en nucleocytoplasmatische transporte...
1. PREPAREREN EN OBSERVEREN VAN CELLEN EN
WEEFSELS
1.1. INLEIDING
doel: cellen en weefsels observeerbaar maken voor LM of EM
probleem:
o biologisch materiaal meestal te dik om zowel licht als elektronen door te laten
o te weinig contrast om structuurdetails te kunnen waarnemen
methodes:
o fixeren
o inbedden
o snijden
o kleuren of contrasteren
1.2. FIXEREN
1.2.1. PROBLEEMSTELLING
weefselstukje wordt uit zijn normale in vivo situatie weggenomen
activatie afbraakenzymen
degradatie van het weefsel
oplossing: denaturatie van eiwitten 2 manieren
o chemische fixatiemiddelen
o bevriezen van het weefsel
1.2.2. CHEMISCHE FIXATIEMIDDELEN
soorten:
o LM: formol
o EM: glutaraldehyde, OSO4
methode: eiwitten crosslinken (met formol of glutaraldehyde) of neerslaan (met
alcohol of aceton)
gevolg:
1
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