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Samenvatting Cytologie: Hoofdstuk 1 (Techniek) $3.25   Add to cart

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Samenvatting Cytologie: Hoofdstuk 1 (Techniek)

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Samenvatting in puntjes van hoofdstuk 1 (Techniek) van cytologie (cel 2). De documenten die hierop volgen zijn: - Cytologie: Hoofdstuk 2 (De cel-veel kostbaar goeds in een fragiele verpakking) - Cytologie: Hoofdstuk 3 (Bloed) - Cytologie: Hoofdstuk 4 (De nucleus en nucleocytoplasmatische transporte...

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  • January 16, 2018
  • 19
  • 2017/2018
  • Summary
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Hoofdstuk 1: Techniek

1. PREPAREREN EN OBSERVEREN VAN CELLEN EN
WEEFSELS

1.1. INLEIDING

 doel: cellen en weefsels observeerbaar maken voor LM of EM

 probleem:
o biologisch materiaal meestal te dik om zowel licht als elektronen door te laten
o te weinig contrast om structuurdetails te kunnen waarnemen

 methodes:
o fixeren
o inbedden
o snijden
o kleuren of contrasteren




1.2. FIXEREN

1.2.1. PROBLEEMSTELLING

 weefselstukje wordt uit zijn normale in vivo situatie weggenomen
 activatie afbraakenzymen
 degradatie van het weefsel
 oplossing: denaturatie van eiwitten  2 manieren
o chemische fixatiemiddelen
o bevriezen van het weefsel




1.2.2. CHEMISCHE FIXATIEMIDDELEN

 soorten:
o LM: formol
o EM: glutaraldehyde, OSO4

 methode: eiwitten cross­linken (met formol of glutaraldehyde) of neerslaan (met
alcohol of aceton)

 gevolg:



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