100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Samenvatting Cytologie: Hoofdstuk 1 (Techniek) $3.24   Add to cart

Summary

Samenvatting Cytologie: Hoofdstuk 1 (Techniek)

 143 views  0 purchase
  • Course
  • Institution

Samenvatting in puntjes van hoofdstuk 1 (Techniek) van cytologie (cel 2). De documenten die hierop volgen zijn: - Cytologie: Hoofdstuk 2 (De cel-veel kostbaar goeds in een fragiele verpakking) - Cytologie: Hoofdstuk 3 (Bloed) - Cytologie: Hoofdstuk 4 (De nucleus en nucleocytoplasmatische transporte...

[Show more]

Preview 1 out of 19  pages

  • January 16, 2018
  • 19
  • 2017/2018
  • Summary
avatar-seller
Hoofdstuk 1: Techniek

1. PREPAREREN EN OBSERVEREN VAN CELLEN EN
WEEFSELS

1.1. INLEIDING

 doel: cellen en weefsels observeerbaar maken voor LM of EM

 probleem:
o biologisch materiaal meestal te dik om zowel licht als elektronen door te laten
o te weinig contrast om structuurdetails te kunnen waarnemen

 methodes:
o fixeren
o inbedden
o snijden
o kleuren of contrasteren




1.2. FIXEREN

1.2.1. PROBLEEMSTELLING

 weefselstukje wordt uit zijn normale in vivo situatie weggenomen
 activatie afbraakenzymen
 degradatie van het weefsel
 oplossing: denaturatie van eiwitten  2 manieren
o chemische fixatiemiddelen
o bevriezen van het weefsel




1.2.2. CHEMISCHE FIXATIEMIDDELEN

 soorten:
o LM: formol
o EM: glutaraldehyde, OSO4

 methode: eiwitten cross­linken (met formol of glutaraldehyde) of neerslaan (met
alcohol of aceton)

 gevolg:



1

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller geneeskundeftpsychologie. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $3.24. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

80796 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$3.24
  • (0)
  Add to cart