Class notes
Enzymology in a nutshell
- Course
- Institution
- Book
Hand written notes on enzymology , foundation of biochemistry and basics of enzymes
[Show more]
Connected book
Book Title:
Author(s):
- Edition:
- ISBN:
- Edition:
More summaries for
-
Hypoglycaemia
-
Glycolysis pathway
-
Diabetes
Seller
Follow
samruddhibhalekar
Content preview
what areEzylog
?
or enzymes
,
enzymes
d ↳ specialised proteins
&
-
Biocatalysts ↳ speed up the chemical reaction
Zu ↑ ↳largely predominate in
What is a
catalyst ? 2 inorganic
ions
of that
increase/decrease
Marietyatalysts
② catalyst The rate of reaction
but not all catals in
are
enzymes .
V
Differences Catalyst
Enzyme
-
#
&
-
I . -
organic
in nature Based on -Inorganic in nature
2.
High molecular notchemical low Molecular weight
-
-
Nature metal ions
gobular proteins
.
3 - Are "proteins" - Are Metal Ions/ enzyme
4 There
. are a types : Based on There are a types
1. Activationenzymes types
. 1 . Positive catalysts
2 .
Inhibitory enzymes .
2 Negative catalysts
.
.Gives
5 faster late of Based on
Gives slower rate of
reaction . Rate of R
. reaction
aged .
-largely remain
. Enzymes need
6 optimum Based on Catalysts do not need
temp pH soll
:
, they temperature any
,
optimum temp ph ,
are
specialised proteins PH came are stable at all
tery
S
.
,
act diff.
enence work
,
easily
PH .
(highes/lawer).
i e
.
secific denatures Anything !!I
.
7
Enzymes are more Based on Catalysts are less
specific specificity
.
specific comp. to enzym
, O catalysts
similarity of
enzymes
↓
⑧samsuddtiene
Both are not consumed in the
estion ?? entire course of reaction .
Approve / Disapprove :
1
II
All are
protein in
enzymes nature
No ,
I
disapprove
, Not "All" enzymes are
protein in nature
,
There's an
exception :
Ribozymes a made of
up
RNA
An consists of 2
portions parts /
enzyme
.
-
Apenzyme cofactor/coenzyme
-
↓
-
↓
-
d organic part
of non-protein
protein part I is
part a A is derived
from
↓ enzyme
an
metal ion
V
Vitamins"
My 44tca2+
+
But is an catalytic +
inactive portion . 2n
Wi no cofactor att to it
.
Agloenzyme what makes an catalytic enzyme
the way
,
↳
Catalytic active enz. it is ?
↳ makes the
i
.
capable of converting
e Presence of cofactor enzyme
the substrate to prod catalytic active . N
sout of an requirement for
a bounded
cofactors
&
catalytic active
.
to it
.
Move about cofactors
types
--
: Are vitamins ORGANIC IN ORGANIC
in nature
Vitamin B complex
6Coenzymes ↓ ↓
metalions
eg : Riboflavin
2/ help of &
These
coenzymes/lofactors are fightly bound
enzyme (Apoenzyme)
to the are are
COVALENTING termed as "Prosthetic groups"
, &
co-substrate is the one cofactor
A
Inon-protein
+
Apoenzyme
(protein part)
i. e not
rightly bounded to part) H
MOLOENZYME
·
the
enzyme
.
Lactive enzyme)
&
DIEF UNIIS TO MEASURE ENZYE
S ACTIVITY us rate of conversion of
-
substrate to
product
Enzymes
.
:
International Unit (IV] are biomassess -> The
change in
S helps us to
their levels (as
umol/min understand they are
protein)
↓ what's next to
Amt of
enzyme that catalyses come .
I mol of
the conversion of
substrate
per min under
->
Revised -
Kata
- a
katal of
I enzyme
Optimum conditions (pH temp
, ,
con!
activity converts (mol of
substrate per second .
1 IV 16 6 7 n katal i nmol/s
to
we know =
.
&
.
e
= 16 . 67
· 2I0=2x10 67 .
n Katal
= 43 34 .
n Katal
/
Activity Turnover number
Specific - -
of of substrate molecules
->
Activity an
enzyme per -> no
converted
mg of an total protein
. to
product per
↓ .
second
Units : mol of substrate converted
Inactive form of an
zymogen :
into product per min/mg enzyme
similar
under optimum condition
:
pepsinogen
d chymotrypsinogen .
: . mol of substrate/min/mg
impo d
↳
use
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller samruddhibhalekar. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
50843 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now