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AAS-analyse: bepaling van Ca2+ in een serum1. Doel
Bepaling van de concentratie Ca in een onbekend serumstaal (uitgedrukt in ppm) a.d.h.v
AAS-analyse m.b.v. de externe standaard ijklijnmethode.
2. Principe
Analysemethode: Atomaire absorptie-spectrofotometrie
Bepalingsmethode: Externe standaard-ijklijnmethode
Formules:
Formule Legende
Verdunningsregel: V = geconcentreerde oplossing in mL
C = geconcentreerde oplossing in ppm
C ∙ V =C ' ∙ V ' V’= verdunde oplossing in mL
C’= verdunde oplossing in ppm
Externe standaard ijklijn: y = piekhoogte
y = ax +b x = concentratie in ppm
Variatiecoëfficiënt: SD = standaardafwijking van de metingen
SD x = gemiddelde van de concentratie in ppm
VC% = ∙ 100%
x
3. Materiaal
Toestellen Chemicaliën Materiaal
PinAAcle 500; Ca2+-stockoplossing Pipetpunten
PerkinElmer, 100,0 ppm 2x maatkolf 20,00mL
00084053 La3+- oplossing 1% 3x maatkolf 50,00 mL
Analytische balans HCL (37%) 1x maatkolf 100,0mL
Semi-automatische Serumstaal 3x maatbeker
micropipet Gedemineraliseerd 50,00mL
water Plastic potjes
4. Werkwijze
Zie labnota’s ‘Labcluster Instrumentele Analyse 2’ pagina 6
Meetvoorwaarden: = 422,67
Split width = 0,7 nm
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