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Essay

Practicum 4 Ethanol

1 review
 594 views  8 purchases
  • Course
  • Moleculen
  • Institution
  • Universiteit Utrecht (UU)

Volledige uitwerking van het practicum met alcohol: het alcoholgehalte in bloed wordt bepaald via een enzymatische substraatbepaling. Er zijn verschillende stoffen toegevoegd waarvan het effect op het alcoholgehalte wordt bepaald.

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Document information

  • April 2, 2018
  • 4
  • 2017/2018
  • Essay
  • Unknown
  • 7,5

Subjects

  • alcohol
  • bloed
  • promille
  • promillage
  • propanol
  • plot
  • enzymatisch
  • bepaling
  • substraat

Written for

  • Universiteit Utrecht (UU)
  • Biomedische Wetenschappen
  • Moleculen
All documents for this subject (78)

1  review

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By: giadakruidenier • 4 year ago

Seller

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michelleweesie

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Content preview

Practicum 4: Enzymatische bepaling van alcohol in
bloed
Door Rosa Wassenaar (6093345, groep 9) en Michelle Weesie
(6157904, groep 10)
Dhr. B.M. Gadella
Assistent: Dorien, Lisa

In dit practccu wordt het alcoholpercentage in bloed bepaald via een enzyuatichee-
icbitraatbepaling. Het icbitraat in het uoniter wordt volledig ougezet door het enzyu
alcohol dehydrogenaie ADH.

Resultaten
Ccvet 10: Bloed + 6,0‰
Tijd Abiorpte: A340
(uincten)
0 0,075
5 0,380
10 0,594
15 0,727
20 0,818
25 0,883
30 0,932
Tabel 1: Bepaling A340 van cuvet 10 om de 5 minuten.


Ccvet 10: Bloed + 0,6% ethanol A340 citgezet tegen tjd
1.000
0.900
0.800
0.700
0.600
A 340 nu




0.500
0.400
0.300
0.200
0.100
0.000
0 5 10 15 20 25 30
Tijd in uincten

Grafee 1: Curve van A340 uitgezet tegen de tid van 0 tot 30 minuten.

 Vlakt de ccrve af?

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