ASCP MB EXAM
QUESTIONS AND
CORRECT ANSWERS
RATED A+/ ASCP MB
EXAM LATEST 2023/2024
Predominant form of DNA - ANSWERS-Right-handed B
DNA and histone interaction is due to what type of bond? - ANSWERS-Ionic
What stains to the minor groove of DNA duplex? - ANSWERS-SYBR Green ...
ASCP MB EXAM
QUESTIONS AND
CORRECT ANSWERS
RATED A+/ ASCP MB
EXAM LATEST 2023/2024
Predominant form of DNA - ANSWERS-Right-handed B
DNA and histone interaction is due to what type of bond? - ANSWERS-Ionic
What stains to the minor groove of DNA duplex? - ANSWERS-SYBR Green
What is the most abundant type of RNA? - ANSWERS-Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - ANSWERS-Genome and mRNA
What increases half-life of mRNA? - ANSWERS-5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - ANSWERS-Euchromatin
What is the most common type of eukaryotic DNA? - ANSWERS-Satellite DNA
,What specimen has the most DNA according to molecular weight? - ANSWERS-White
Blood Cells
What amino acids compose histones? - ANSWERS-Lysine and Arginine
Amino acid that all proteins start with: - ANSWERS-Methionine
What protects the DNA strand during replication? - ANSWERS-Single-stranded binding
protein
Leading Strand - ANSWERS-3' to 5' template
Lagging Strand - ANSWERS-5' to 3' template
What DNA strand is being copied in transcription? - ANSWERS-Antisense Strand
First step after newly formed mRNA is made? - ANSWERS-Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
ANSWERS-Telomerase
What polymerase has repair activity? - ANSWERS-Beta/Epsilon
What polymerase acts on leading strand? - ANSWERS-Delta
What polymerase acts on lagging strand? - ANSWERS-Alpha
Which polymerase is used for mitochondrial replication and repair? - ANSWERS-
Gamma
What polymerase binds the 5' end to 3' end of dsDNA? - ANSWERS-Topoisomerase
, Which RNA polymerase is mainly for transcription? - ANSWERS-RNA Pol II
Which polymerase is involved with mRNA synthesis? - ANSWERS-RNA Pol II
What inactivates RNAses? - ANSWERS-Diethyl pyrocarbonate (DEPC) or bake
glassware for 4 to 6 hours at 400C
What type of restriction enzyme recognizes palindromic sites? - ANSWERS-Restriction
Enzyme Type II
What type of restriction enzyme lacks methylation activity? - ANSWERS-Restriction
Enzyme Type II
What is necessary to make cDNA? - ANSWERS-Reverse transcriptase
Number of SNPs in a human genome? - ANSWERS-11 billion (10^7)
How many genes (base pairs) are in a human haploid genome? - ANSWERS-3 billion
base pairs
The polymerization of acrylamide requires: - ANSWERS-Catalyst (APS or TEMED) or
Light activation
How does agarose gel pore size relate to concentration? - ANSWERS-Inversely
proportional
What is denatured during a southern blot? - ANSWERS-Hydrogen bonds (inter-strand
bonds)
Melting temperature formula - ANSWERS-Tm = (4C x GC Pairs) + (2C x AT pairs)
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