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Exam (elaborations)

BIOL202 Week 7 Lab 7 Differential and Biochemical Tests APU

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 Each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit  If the question asks you to do research or find a source, a reputable, credible and/or scholarly source citation must be included in order to be considered for full credit  If a math formula is required to arrive to an answer, work must be shown otherwise, no credit will be awarded Experiment3ResultsTables  Table3:ZoneofInhibitionforSkinSample(10points)  Table4:ZoneofInhibitionforLocalSample(10points)  Table5:ZoneofInhibitionforYeastSample(10points) Experiment3Post-LabQuestions 1. Table 6 outlines interpretive standards of antibiotic resistance based on the size ofinhibition zones. For example, many bacterial species that are resistant to Kanamycinhave an inhibition zone of 13 mm or smaller. Of course, inhibition zones may also beaffectedbytheantibiotic concentrationpresent withinthe disk. Use Table 6 to determine whether the microbial samples you tested are sensitive (S),moderatelyresistant(MR),orresistant(R)toeachoftheantibioticdisks.Remember,you will not know the exact species of bacteria on your “Skin” and “Local” plates. Thetableis a guidetocompare yourresults toastandard. Describe the details of each of your sample plates growth morphology, the diameter ofthe zones for each antibiotic and whether or not they were sensitive (S), moderatelysensitive(MR)orresistant (R) basedonthezonesyouobserved(20points) 2. Is the bacterial lawn sampled from your skin more resistant to one antibiotic than theothers? What about the local sample? The yeast sample? Use the detailed results fromQuestion1tosupportyouranswer.(10points) 3. What is the significance of the size of the zone of inhibition with respect to the microbesamples growth on the plates? (10 points) . 4. Research and describe the relationship between the minimum inhibitory concentration(MIC)andthezoneofinhibition.DeterminetheMICandtheeffectivenessoftheantibiotics used in your experiments. Note, disk potencies are listed per antibiotic in theMaterialssectionbeforetheexperimentalprocedures.(20points) 5. Use a reputable internet source to determine and describe which cellular component inthebacterialcellistargetedbyeachoftheantibioticsusedinthisexperiment.(10points).

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  • biol202 week 7 lab 7

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